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Autoren: Langendorf, Eva K.
Rommens, Pol M.
Drees, Philipp
Mattyasovszky, Stefan G.
Ritz, Ulrike
Titel: Detecting the effects of the glucocorticoid dexamethasone on primary human skeletal muscle cells : differences to the murine cell line
Online-Publikationsdatum: 28-Aug-2020
Sprache des Dokuments: Englisch
Zusammenfassung/Abstract: Skeletal muscle atrophy is characterized by a decrease in muscle fiber size as a result of a decreased protein synthesis, which leads to degradation of contractile muscle fibers. It can occur after denervation and immobilization, and glucocorticoids (GCs) may also increase protein breakdown contributing to the loss of muscle mass and myofibrillar proteins. GCs are already used in vitro to induce atrophic conditions, but until now no studies with primary human skeletal muscle existed. Therefore, this study deals with the effects of the GC dexamethasone (dex) on primary human myoblasts and myotubes. After incubation with 1, 10, and 100 µM dex for 48 and 72 h, gene and protein expression analyses were performed by qPCR and Western blot. Foxo, MuRF-1, and MAFbx were significantly upregulated by dex, and there was increased gene expression of myogenic markers. However, prolonged incubation periods demonstrated no Myosin protein degradation, but an increase of MuRF-1 expression. In conclusion, applying dex did not only differently affect primary human myoblasts and myotubes, as differences were also observed when compared to murine cells. Based on our findings, studies using cell lines or animal cells should be interpreted with caution as signaling transduction and functional behavior might differ in diverse species. Keywords: atrophy; MuRF-1; myotubes; myoblasts; MAFbx; glucocorticoids; dexamethasone; Foxo; Myosin
DDC-Sachgruppe: 540 Chemie
540 Chemistry and allied sciences
570 Biowissenschaften
570 Life sciences
610 Medizin
610 Medical sciences
Veröffentlichende Institution: Johannes Gutenberg-Universität Mainz
Organisationseinheit: FB 04 Medizin
Veröffentlichungsort: Mainz
DOI: http://doi.org/10.25358/openscience-5091
Version: Published version
Publikationstyp: Zeitschriftenaufsatz
Weitere Angaben zur Dokumentart: Scientific article
Nutzungsrechte: CC-BY
Informationen zu den Nutzungsrechten: https://creativecommons.org/licenses/by/4.0/
Zeitschrift: International journal of molecular sciences
21
7
Seitenzahl oder Artikelnummer: 2497
Verlag: MDPI
Verlagsort: Basel
Erscheinungsdatum: 2020
ISSN: 1422-0067
URL der Originalveröffentlichung: https://doi.org/10.3390/ijms21072497
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