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http://doi.org/10.25358/openscience-5091
Autoren: | Langendorf, Eva K. Rommens, Pol M. Drees, Philipp Mattyasovszky, Stefan G. Ritz, Ulrike |
Titel: | Detecting the effects of the glucocorticoid dexamethasone on primary human skeletal muscle cells : differences to the murine cell line |
Online-Publikationsdatum: | 28-Aug-2020 |
Erscheinungsdatum: | 2020 |
Sprache des Dokuments: | Englisch |
Zusammenfassung/Abstract: | Skeletal muscle atrophy is characterized by a decrease in muscle fiber size as a result of a decreased protein synthesis, which leads to degradation of contractile muscle fibers. It can occur after denervation and immobilization, and glucocorticoids (GCs) may also increase protein breakdown contributing to the loss of muscle mass and myofibrillar proteins. GCs are already used in vitro to induce atrophic conditions, but until now no studies with primary human skeletal muscle existed. Therefore, this study deals with the effects of the GC dexamethasone (dex) on primary human myoblasts and myotubes. After incubation with 1, 10, and 100 µM dex for 48 and 72 h, gene and protein expression analyses were performed by qPCR and Western blot. Foxo, MuRF-1, and MAFbx were significantly upregulated by dex, and there was increased gene expression of myogenic markers. However, prolonged incubation periods demonstrated no Myosin protein degradation, but an increase of MuRF-1 expression. In conclusion, applying dex did not only differently affect primary human myoblasts and myotubes, as differences were also observed when compared to murine cells. Based on our findings, studies using cell lines or animal cells should be interpreted with caution as signaling transduction and functional behavior might differ in diverse species. Keywords: atrophy; MuRF-1; myotubes; myoblasts; MAFbx; glucocorticoids; dexamethasone; Foxo; Myosin |
DDC-Sachgruppe: | 540 Chemie 540 Chemistry and allied sciences 570 Biowissenschaften 570 Life sciences 610 Medizin 610 Medical sciences |
Veröffentlichende Institution: | Johannes Gutenberg-Universität Mainz |
Organisationseinheit: | FB 04 Medizin |
Veröffentlichungsort: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-5091 |
Version: | Published version |
Publikationstyp: | Zeitschriftenaufsatz |
Weitere Angaben zur Dokumentart: | Scientific article |
Nutzungsrechte: | CC BY |
Informationen zu den Nutzungsrechten: | https://creativecommons.org/licenses/by/4.0/ |
Zeitschrift: | International journal of molecular sciences 21 7 |
Seitenzahl oder Artikelnummer: | 2497 |
Verlag: | MDPI |
Verlagsort: | Basel |
Erscheinungsdatum: | 2020 |
ISSN: | 1422-0067 |
URL der Originalveröffentlichung: | https://doi.org/10.3390/ijms21072497 |
DOI der Originalveröffentlichung: | 10.3390/ijms21072497 |
Enthalten in den Sammlungen: | JGU-Publikationen |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | ||
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langendorf_eva_k.-detecting_the_-20200820172623600.pdf | 4.43 MB | Adobe PDF | Öffnen/Anzeigen |