Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-2894
Authors: | Unichenko, Petr |
Title: | Extracellular glutamate-GABA balance in the developing neocortex |
Online publication date: | 14-Oct-2014 |
Year of first publication: | 2014 |
Language: | english |
Abstract: | It has been shown in the study that glutamate transporters (EAAT) are capable to modulate GABA transports (GAT). Here we also report that DL-TBOA, a non-transportable glutamate uptake blocker, eliminates GAT-mediated GABA release, while D-aspartate, an EAAT substrate, does not block the latter. The strength or even the operating mode of GABA uptake/release could be influenced by the work of EAATs. Considering the interaction between EAATs and GATs we can conclude that ambient glutamate and GABA levels are mutually dependent. The EAAT-GAT crosstalk observed in this work is mediated by EAAT1 and GAT-2/3. Since both transporters are Na+ dependent and mainly glial, next we investigated the role of [Na+]i in astrocytic-mediated glutamate uptake. We tested whether [Na+]i changes affect paired-pulse plasticity of STCs recorded from cortical layer 2/3 astrocytes. We report that an elevation of [Na+]i induced either by using a high [Na+]i intrapipette solution or by application of GABA slows STCs kinetics and decrease paired-pulse facilitation (PPF) of STCs at short inter-stimulus intervals. Moreover, GAT inhibitors decrease PPF of STCs under control conditions, suggesting that endogenous GABA operating via GATs influences EAAT-mediated transport |
DDC: | 570 Biowissenschaften 570 Life sciences |
Institution: | Johannes Gutenberg-Universität Mainz |
Department: | FB 04 Medizin |
Place: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-2894 |
URN: | urn:nbn:de:hebis:77-38645 |
Version: | Original work |
Publication type: | Dissertation |
License: | In Copyright |
Information on rights of use: | https://rightsstatements.org/vocab/InC/1.0/ |
Extent: | 95 Bl. |
Appears in collections: | JGU-Publikationen |