Authors:	Laspas, Panagiotis Zhutdieva, Mayagozel B. Brochhausen, Christoph Musayeva, Aytan Zadeh, Jenia Kouchek Pfeiffer, Norbert Xia, Ning Li, Huige Wess, Jürgen Gericke, Adrian
Title:	The M1 muscarinic acetylcholine receptor subtype is important for retinal neuron survival in aging aging mice
Online publication date:	11-Jul-2019
Year of first publication:	2019
Language:	english
Abstract:	Muscarinic acetylcholine receptors have been implicated as potential neuroprotective targets for for glaucoma. We tested the hypothesis that the lack of a single muscarinic receptor subtype leads to to age-dependent neuron reduction in the retinal ganglion cell layer. Mice with targeted disruption of of single muscarinic acetylcholine receptor subtype genes (M1 to M5) and wild-type controls were examined examined at two age categories, 5 and 15 months, respectively. We found no differences in intraocular intraocular pressure between individual mouse groups. Remarkably, in 15-month-old mice devoid of the the M1 receptor, neuron number in the retinal ganglion cell layer and axon number in the optic nerve were markedly reduced. Moreover, mRNA expression for the prooxidative enzyme, NOX2, was increased increased, while mRNA expression for the antioxidative enzymes, SOD1, GPx1 and HO-1, was reduced in aged aged M1 receptor-deficient mice compared to age-matched wild-type mice. In line with these findings findings, the reactive oxygen species level was also elevated in the retinal ganglion cell layer of aged aged M1 receptor-deficient mice. In conclusion, M1 receptor deficiency results in retinal ganglion cell cell loss in aged mice via involvement of oxidative stress. Based on these findings, activation of M1 receptor signaling may become therapeutically useful to promote retinal ganglion cell survival.
DDC:	610 Medizin 610 Medical sciences
Institution:	Johannes Gutenberg-Universität Mainz
Department:	FB 04 Medizin
Place:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-179
Version:	Published version
Publication type:	Zeitschriftenaufsatz
License:	CC BY
Information on rights of use:	https://creativecommons.org/licenses/by/4.0/
Journal:	Scientific reports 9
Pages or article number:	Art. 5222
Publisher:	Macmillan Publishers Limited, part of Springer Nature
Publisher place:	London
Issue date:	2019
ISSN:	2045-2322
Publisher URL:	http://dx.doi.org/10.1038/s41598-019-41425-5
Publisher DOI:	10.1038/s41598-019-41425-5
Appears in collections:	JGU-Publikationen