Genomanalyse von Prodiamesa olivacea
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Abstract
In this work, with the help of high-throughput sequencing, a partial analysis of the ~113 Mb-sized Prodiames olivacea genome was achieved. About ~74Mb (~ 14000 genes) could be covered in the process. Aside from highly and moderately repetitive DNA sequences such as satellite-DNA and histone- or hemoglobin genes, homologous genes from the sex determination cascade of Drosophila melanogaster were analyzed. Only one of the genes, the gene doublesex (dsx), has a probable role in the sex determination process. The in-frame-intron of the dsx-homologous gene represents a special case. This intron which has been identified as mosquito-specific, has also been found in P. olivacea and Chironomus -species. After analyzing the splice acceptor- and donor-sites, a splicing mechanism of dsx in P. olivacea as well as Anopheles gambiae is most probable. Additionally, an orthologous dsx-gene could be identified in P. olivacea. Previously, this could only be achieved for the three species of mosquito, as well as in some sub-species such as Chironomus. Since a Southern-analysis found no duplication for the gene fs(1)K10-like in P. olivacea male, as