Authors:	Clausen, Björn E. Amon, Lukas Backer, Ronald A. Berod, Luciana Bopp, Tobias Brand, Anna Burgdorf, Sven Chen, Luxia Da, Meihong Distler, Ute Dress, Regine J. Dudziak, Diana Dutertre, Charles-Antoine Eich, Christina Gabele, Anna Geiger, Melanie Ginhoux, Florent Giusiano, Lucila Godoy, Gloria J. Hamouda, Ahmed E.I. Hatscher, Lukas Heger, Lukas Heidkamp, Gordon F. Hernandez, Lola C. Jacobi, Lukas Kaszubowski, Tomasz Kong, Wan Ting Lehmann, Christian H. K. López-López, Tamara Mahnke, Karsten Nitsche, Dominik Renkawitz, Jörg Reza, Rifat A. Sáez, Pablo J. Schlautmann, Laura Schmitt, Madeleine T. Tenzer, Stefan Tochoedo, Nounagnon R. Vurnek, Damir Zink, Fabian Hieronymus, Thomas
Title:	Guidelines for mouse and human DC functional assays
Online publication date:	7-Mar-2024
Year of first publication:	2022
Language:	english
Abstract:	This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state-of-the-art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs and various non-lymphoid tissues. Recent studies have provided evidence for an increasing number of phenotypically distinct conventional DC (cDC) subsets that on one hand exhibit a certain functional plasticity, but on the other hand are characterized by their tissue- and context-dependent functional specialization. Here, we describe a selection of assays for the functional characterization of mouse and human cDC. The first two protocols illustrate analysis of cDC endocytosis and metabolism, followed by guidelines for transcriptomic and proteomic characterization of cDC populations. Then, a larger group of assays describes the characterization of cDC migration in vitro, ex vivo, and in vivo. The final guidelines measure cDC inflammasome and antigen (cross)-presentation activity. While all protocols were written by experienced scientists who routinely use them in their work, this article was also peer-reviewed by leading experts and approved by all co-authors, making it an essential resource for basic and clinical DC immunologists.
DDC:	610 Medizin 610 Medical sciences
Institution:	Johannes Gutenberg-Universität Mainz
Department:	FB 04 Medizin
Place:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-10167
Version:	Published version
Publication type:	Zeitschriftenaufsatz
Document type specification:	Other
License:	CC BY
Information on rights of use:	https://creativecommons.org/licenses/by/4.0/
Journal:	European journal of immunology 53 2
Pages or article number:	2249925
Publisher:	Wiley
Publisher place:	Weinheim
Issue date:	2022
ISSN:	1521-4141
Publisher DOI:	10.1002/eji.202249925
Appears in collections:	DFG-491381577-H