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Authors: Sun, Mo
Deng, Jie
Walther, Andreas
Title: Communication and cross-regulation between chemically fueled sender and receiver reaction networks
Online publication date: 1-Mar-2024
Year of first publication: 2022
Language: english
Abstract: Nature connects multiple fuel-driven chemical/enzymatic reaction networks (CRNs/ERNs) via cross-regulation to hierarchically control biofunctions for a tailored adaption in complex sensory landscapes. Herein, we introduce a facile example of communication and cross-regulation among two fuel-driven DNA-based ERNs regulated by a concatenated RNA transcription regulator. ERN1 (“sender”) is designed for the fuel-driven promoter formation for T7 RNA polymerase, which activates RNA transcription. The produced RNA can deactivate or activate DNA in ERN2 (“receiver”) by toehold-mediated strand displacement, leading to a communication between two ERNs. The RNA from ERN1 can repress or promote the fuel-driven state of ERN2; ERN2 in turn feedbacks to regulate the lifetime of ERN1. Furthermore, the incorporation of RNase H allows for RNA degradation and enables the autonomous recovery of ERN2. We believe that concatenation of multiple CRNs/ERNs provides a basis for the design of more elaborate autonomous regulatory mechanisms in systems chemistry and synthetic biology.
DDC: 540 Chemie
540 Chemistry and allied sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 09 Chemie, Pharmazie u. Geowissensch.
Place: Mainz
Version: Published version
Publication type: Zeitschriftenaufsatz
Document type specification: Scientific article
License: CC BY-NC
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Journal: Angewandte Chemie International Edition
Pages or article number: e202214499
Publisher: Wiley
Publisher place: Weinheim
Issue date: 2022
ISSN: 1521-3773
Publisher DOI: 10.1002/anie.202214499
Appears in collections:DFG-491381577-H

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