Investigation of the electric field enhanced PNA-DNA hybridization on Au surface by using surface plasmon field-enhanced fluorescence spectroscopy (SPFS)

dc.contributor.authorJeon, Hyunpyo
dc.date.accessioned2011-02-15T15:31:56Z
dc.date.available2011-02-15T16:31:56Z
dc.date.issued2011
dc.description.abstractRecently, the surface plasmon field-enhanced fluorescence spectroscopy (SPFS) was developed as a kinetic analysis and a detection method with dual- monitoring of the change of reflectivity and fluorescence signal for the interfacial phenomenon. A fundamental study of PNA and DNA interaction at the surface using surface plasmon fluorescence spectroscopy (SPFS) will be investigated in studies. Furthermore, several specific conditions to influence on PNA/DNA hybridization and affinity efficiency by monitoring reflective index changes and fluorescence variation at the same time will be considered. In order to identify the affinity degree of PNA/DNA hybridizaiton at the surface, the association constant (kon) and the dissociation constant (koff) will be obtained by titration experiment of various concentration of target DNA and kinetic investigation. In addition, for more enhancing the hybridization efficiency of PNA/DNA, a study of polarized electric field enhancement system will be introduced and performed in detail. DNA is well-known polyelectrolytes with naturally negative charged molecules in its structure. With polarized electrical treatment, applying DC field to the metal surface, which PNA probe would be immobilized at, negatively charged DNA molecules can be attracted by electromagnetic attraction force and manipulated to the close the surface area, and have more possibility to hybridize with probe PNA molecules by hydrogen bonding each corresponding base sequence. There are several major factors can be influenced on the hybridization efficiency.en_GB
dc.identifier.doihttp://doi.org/10.25358/openscience-3287
dc.identifier.urihttps://openscience.ub.uni-mainz.de/handle/20.500.12030/3289
dc.identifier.urnurn:nbn:de:hebis:77-26804
dc.language.isoeng
dc.rightsInC-1.0de_DE
dc.rights.urihttps://rightsstatements.org/vocab/InC/1.0/
dc.subject.ddc540 Chemiede_DE
dc.subject.ddc540 Chemistry and allied sciencesen_GB
dc.titleInvestigation of the electric field enhanced PNA-DNA hybridization on Au surface by using surface plasmon field-enhanced fluorescence spectroscopy (SPFS)en_GB
dc.typeDissertationde_DE
jgu.description.extent131 S.
jgu.organisation.departmentFB 09 Chemie, Pharmazie u. Geowissensch.
jgu.organisation.nameJohannes Gutenberg-Universität Mainz
jgu.organisation.number7950
jgu.organisation.placeMainz
jgu.organisation.rorhttps://ror.org/023b0x485
jgu.organisation.year2011
jgu.rights.accessrightsopenAccess
jgu.subject.ddccode540
jgu.type.dinitypePhDThesis
jgu.type.resourceText
jgu.type.versionOriginal worken_GB
opus.date.accessioned2011-02-15T15:31:56Z
opus.date.available2011-02-15T16:31:56
opus.date.modified2011-02-28T09:31:33Z
opus.identifier.opusid2680
opus.institute.number0905
opus.metadataonlyfalse
opus.organisation.stringFB 09: Chemie, Pharmazie und Geowissenschaften: Institut für Organische Chemiede_DE
opus.subject.dfgcode00-000
opus.subject.othersurface plasmon, SPFS, biosensor, PNA, hybridizationen_GB
opus.type.contenttypeDissertationde_DE
opus.type.contenttypeDissertationen_GB

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