Mobilization of CD34+-progenitor cells in patients with severe trauma

dc.contributor.authorRitz, Ulrike
dc.contributor.authorSpies, Volker
dc.contributor.authorMehling, Isabella
dc.contributor.authorGruszka, Dominik
dc.contributor.authorRommens, Pol Maria
dc.contributor.authorHofmann, Alexander
dc.date.accessioned2022-10-05T10:32:37Z
dc.date.available2022-10-05T10:32:37Z
dc.date.issued2014
dc.description.abstractCirculating CD34+ progenitor cells () gained importance in the field of regenerative medicine due to their potential to home in on injury sites and differentiate into cells of both endothelial and osteogenic lineages. In this study, we analyzed the mobilization kinetics and the numbers of CD34+, CD31+, CD45+, and CD133+ cells in twenty polytrauma patients (n = 13 male, n = 7 female, mean age 46.5±17.2 years, mean injury severity score (ISS) 35.8±12.5 points). In addition, the endothelial differentiation capacity of enriched CD34+cells was assessed by analyzing DiI-ac-LDL/lectin uptake, the expression of endothelial markers, and the morphological characteristics of these cells in Matrigel and spheroid cultures. We found that on days 1, 3, and 7 after a major trauma, the number of CD34+cells increased from 6- up to 12-fold (p<0.0001) over the number of CD34+cells from a control population of healthy, age-matched volunteers. The numbers of CD31+ cells were consistently higher on days 1 (1.4-fold, p<0.01) and 7 (1.3-fold, p<0.01), whereas the numbers of CD133+ cell did not change during the time course of investigation. Expression of endothelial marker molecules in CD34+cells was significantly induced in the polytrauma patients. In addition, we show that the CD34+ cell levels in severely injured patients were not correlated with clinical parameters, such as the ISS score, the acute physiology and chronic health evaluation II score (APACHE II), as well as the sequential organ failure assessment score (SOFA-2). Our results clearly indicate that pro-angiogenic cells are systemically mobilized after polytrauma and that their numbers are sufficient for the development of novel therapeutic models in regenerative medicine.en_GB
dc.description.sponsorshipDFG, Open Access-Publizieren Universität Mainz / Universitätsmedizinde
dc.identifier.doihttp://doi.org/10.25358/openscience-7861
dc.identifier.urihttps://openscience.ub.uni-mainz.de/handle/20.500.12030/7876
dc.language.isoengde
dc.rightsCC-BY-4.0*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subject.ddc610 Medizinde_DE
dc.subject.ddc610 Medical sciencesen_GB
dc.titleMobilization of CD34+-progenitor cells in patients with severe traumaen_GB
dc.typeZeitschriftenaufsatzde
jgu.identifier.pmid24826895
jgu.journal.issue5de
jgu.journal.titlePLoS onede
jgu.journal.volume9de
jgu.organisation.departmentFB 04 Medizinde
jgu.organisation.nameJohannes Gutenberg-Universität Mainz
jgu.organisation.number2700
jgu.organisation.placeMainz
jgu.organisation.rorhttps://ror.org/023b0x485
jgu.pages.alternativee97369de
jgu.publisher.doi10.1371/journal.pone.0097369de
jgu.publisher.issn1932-6203de
jgu.publisher.namePLoSde
jgu.publisher.placeLawrence, Kan.de
jgu.publisher.urihttp://dx.doi.org/10.1371/journal.pone.0097369de
jgu.publisher.year2014
jgu.rights.accessrightsopenAccess
jgu.subject.ddccode610de
jgu.type.dinitypeArticleen_GB
jgu.type.resourceTextde
jgu.type.versionPublished versionde
opus.affiliatedRitz, Ulrike
opus.affiliatedMehling, Isabella
opus.affiliatedGruszka, Dominik
opus.affiliatedRommens, Pol Maria
opus.affiliatedHofmann, Alexander
opus.date.modified2018-08-08T08:56:20Z
opus.identifier.opusid27364
opus.importsourcepubmed
opus.institute.number0439
opus.metadataonlyfalse
opus.organisation.stringFB 04: Medizin: Klinik und Poliklinik für Unfallchirurgiede_DE
opus.subject.dfgcode00-000
opus.type.contenttypeKeinede_DE
opus.type.contenttypeNoneen_EN

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