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http://doi.org/10.25358/openscience-971Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Anaya, Diana Milena | |
| dc.date.accessioned | 2010-09-16T14:27:30Z | |
| dc.date.available | 2010-09-16T16:27:30Z | |
| dc.date.issued | 2010 | |
| dc.identifier.uri | https://openscience.ub.uni-mainz.de/handle/20.500.12030/973 | - |
| dc.description.abstract | The goal of this thesis was to increase the functionality of pristine DNA scaffolds by functionalizing them with fluorescent dyes and hydrophobic moieties. Two important steps were necessary to realize this aim successfully. First, nucleic acids needed to be synthesized making use of multidisciplinary toolbox for the generation and manipulation of polynucleic acids. The most important techniques were the solid phase synthesis involving the incorporation of standard and modified phosphoramidite building blocks as well as molecular biology procedures like the polymerase chain reaction, the bacterial amplification of plasmids and the enzymatic digestion of circular vectors. Second, and evenly important, was the characterization of the novel bioorganic hybrid structures by a multitude of techniques, especially optical measurements. For studying DNA-dye conjugates methods like UV/Vis and photoluminescence spectroscopy as well as time resolved luminescence spectroscopy were utilized. While these measurements characterized the bulk behavior of an ensemble of DNA-dye hybrids it was necessary for a complete understanding of the systems to look at single structures. This was done by single-molecule fluorescence spectroscopy and fluorescence correlation spectroscopy. For complete analysis the optical experiments were complemented by direct visualization techniques, i.e. high resolution transmission electron microscopy and scanning force microscopy. | en_GB |
| dc.description.abstract | Das Ziel dieser Arbeit war die Funktionalität des ursprünglichen DNA-Gerüstes durch Funktionalisierung mit Fluoreszenzfarbstoffen und hydrophoben Einheiten zu erhöhen. Um dieses Ziel erfolgreich zu verwirklichen waren zwei wichtige Schritte notwendig. Zuerst wurden die Nukleinsäuren mit multidisziplinären Techniken synthetisiert. Die wichtigsten Techniken waren die Festphasensynthese mit dem Einbau von Standard -und modifizierte Phosphoramidit Bausteinen sowie molekularbiologische Verfahren wie die Polymerase-Kettenreaktion, die bakterielle Amplifikation von Plasmiden und die enzymatische Restriktion der kreisförmigen Vektoren. Danach, aber nicht weniger von Bedeutung war die Charakterisierung von den bioorganischen hybrid Strukturen durch verschiedene Techniken, insbesondere optischen Messungen. Für das Studium von DNA-Farbstoff-Konjugaten wurden Methoden wie UV/Vis, Photolumineszenz- sowie zeitaufgelöste Lumineszenz-Spektroskopie, Einzel-Molekül-Fluoreszenz-Spektroskopie und Fluoreszenz-Korrelations-Spektroskopie verwendet. | de_DE |
| dc.language.iso | eng | |
| dc.rights | InCopyright | de_DE |
| dc.rights.uri | https://rightsstatements.org/vocab/InC/1.0/ | |
| dc.subject.ddc | 540 Chemie | de_DE |
| dc.subject.ddc | 540 Chemistry and allied sciences | en_GB |
| dc.title | DNA hybrid materials consisting of oligonucleotides and organic molecules: synthesis, characterization and applications | en_GB |
| dc.type | Dissertation | de_DE |
| dc.identifier.urn | urn:nbn:de:hebis:77-24114 | |
| dc.identifier.doi | http://doi.org/10.25358/openscience-971 | - |
| jgu.type.dinitype | doctoralThesis | |
| jgu.type.version | Original work | en_GB |
| jgu.type.resource | Text | |
| jgu.description.extent | 177 S. | |
| jgu.organisation.department | FB 09 Chemie, Pharmazie u. Geowissensch. | - |
| jgu.organisation.year | 2010 | |
| jgu.organisation.number | 7950 | - |
| jgu.organisation.name | Johannes Gutenberg-Universität Mainz | - |
| jgu.rights.accessrights | openAccess | - |
| jgu.organisation.place | Mainz | - |
| jgu.subject.ddccode | 540 | |
| opus.date.accessioned | 2010-09-16T14:27:30Z | |
| opus.date.modified | 2010-09-28T05:24:43Z | |
| opus.date.available | 2010-09-16T16:27:30 | |
| opus.subject.dfgcode | 00-000 | |
| opus.subject.other | DNS, Hybride, Konjugate, Farbstoffe, Hydrophobe Einheiten | de_DE |
| opus.subject.other | DNA, Hybrids, Conjugates, Dyes, Hydrophobic moieties | en_GB |
| opus.organisation.string | FB 09: Chemie, Pharmazie und Geowissenschaften: Institut für Organische Chemie | de_DE |
| opus.identifier.opusid | 2411 | |
| opus.institute.number | 0905 | |
| opus.metadataonly | false | |
| opus.type.contenttype | Dissertation | de_DE |
| opus.type.contenttype | Dissertation | en_GB |
| jgu.organisation.ror | https://ror.org/023b0x485 | |
| Appears in collections: | JGU-Publikationen | |
