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  2. Johannes Gutenberg-Universität Mainz
  3. DFG-491381577-H
Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-9224
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dc.contributor.authorGüler, Baran E.-
dc.contributor.authorLinnert, Joshua-
dc.contributor.authorWolfrum, Uwe-
dc.date.accessioned2023-06-26T10:03:14Z-
dc.date.available2023-06-26T10:03:14Z-
dc.date.issued2023-
dc.identifier.urihttps://openscience.ub.uni-mainz.de/handle/20.500.12030/9241-
dc.description.abstractVLGR1/ADGRV1 (very large G protein-coupled receptor-1) is the largest adhe sion G protein-coupled receptor (aGPCR). Mutations in VLGR1/ADGRV1 are associated with human Usher syndrome, the most common form of deaf blindness, and also with epilepsy in humans and mice. VLGR1 is expressed almost ubiquitously but is mainly found in the CNS and in the sensory cells of the eye and inner ear. Little is known about the pathogenesis of the diseases related to VLGR1. We previously identified VLGR1 as a vital component of focal adhesions (FAs) serving as a metabotropic mechanoreceptor controls cell spreading and migration. FAs are highly dynamic and turnover in response to internal and external signals. Here, we aimed to elucidate how VLGR1 partici pates in FA turnover. Nocodazole washouts and live cell imaging of paxillin DsRed2 consistently showed that FA disassembly was not altered, but de novo assembly of FA was significantly delayed in Vlgr1-deficient astrocytes, indicat ing that VLGR1 is enrolled in FA assembly. In FRAP experiments, recovery rates were significantly reduced in Vlgr1-deficient FAs, indicating reduced turnover kinetics in VLGR1-deficient FAs. We showed that VLGR1 regulates cell migration by controlling the FA turnover during their assembly and expect novel insights into pathomechanisms related to pathogenic dysfunctions of VLGR1.en_GB
dc.language.isoengde
dc.rightsCC BY-NC-ND*
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject.ddc570 Biowissenschaftende_DE
dc.subject.ddc570 Life sciencesen_GB
dc.titleMonitoring paxillin in astrocytes reveals the significance of the adhesion G protein coupled receptor VLGR1/ADGRV1 for focal adhesion assemblyen_GB
dc.typeZeitschriftenaufsatzde
dc.identifier.doihttp://doi.org/10.25358/openscience-9224-
jgu.type.dinitypearticleen_GB
jgu.type.versionPublished versionde
jgu.type.resourceTextde
jgu.organisation.departmentFB 10 Biologiede
jgu.organisation.number7970-
jgu.organisation.nameJohannes Gutenberg-Universität Mainz-
jgu.rights.accessrightsopenAccess-
jgu.journal.titleBasic & clinical pharmacology & toxicologyde
jgu.journal.volumeVersion of Record (VoR)de
jgu.publisher.year2023-
jgu.publisher.nameWiley-Blackwellde
jgu.publisher.placeOxfordde
jgu.publisher.issn1742-7835de
jgu.organisation.placeMainz-
jgu.subject.ddccode570de
dc.date.updated2023-06-07T08:01:37Z-
jgu.publisher.doi10.1111/bcpt.13860de
elements.object.id154232-
elements.object.labelsaGPCR-
elements.object.labelscell migration-
elements.object.labelsepilepsy-
elements.object.labelsfocal adhesions-
elements.object.labelsG protein-coupled receptors-
elements.object.labelsmechanoreception-
elements.object.labelsUsher syndrome-
elements.object.labelsG protein-coupled receptors-
elements.object.labelsUsher syndrome-
elements.object.labelsaGPCR-
elements.object.labelscell migration-
elements.object.labelsepilepsy-
elements.object.labelsfocal adhesions-
elements.object.labelsmechanoreception-
elements.object.labels1115 Pharmacology and Pharmaceutical Sciences-
elements.object.labelsPharmacology & Pharmacy-
elements.object.labels3214 Pharmacology and pharmaceutical sciences-
elements.object.typejournal-article-
jgu.organisation.rorhttps://ror.org/023b0x485-
Appears in collections:DFG-491381577-H

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