Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-8694
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dc.contributor.authorPan, Lili-
dc.contributor.authorTormey, Duncan-
dc.contributor.authorBobon, Nadine-
dc.contributor.authorBaumann, Peter-
dc.date.accessioned2023-01-30T09:20:21Z-
dc.date.available2023-01-30T09:20:21Z-
dc.date.issued2022-
dc.identifier.urihttps://openscience.ub.uni-mainz.de/handle/20.500.12030/8710-
dc.description.abstractThe conserved Rap1 protein is part of the shelterin complex that plays critical roles in chromosome end protection and telomere length regulation. Previous studies have addressed how fission yeast Rap1 contributes to telomere length maintenance, but the mechanism by which the protein inhibits end fusions has remained elusive. Here, we use a mutagenesis screen in combination with high-throughput sequencing to identify several amino acid positions in Rap1 that have key roles in end protection. Interestingly, mutations at these sites render cells susceptible to genome instability in a conditional manner, whereby longer telomeres are prone to undergoing end fusions, while telomeres within the normal length range are sufficiently protected. The protection of long telomeres is in part dependent on their nuclear envelope attachment mediated by the Rap1–Bqt4 interaction. Our data demonstrate that long telomeres represent a challenge for the maintenance of genome integrity, thereby providing an explanation for species-specific upper limits on telomere length.en_GB
dc.description.sponsorshipGefördert durch die Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 491381577de
dc.language.isoengde
dc.rightsCC BY-NC-ND*
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject.ddc570 Biowissenschaftende_DE
dc.subject.ddc570 Life sciencesen_GB
dc.titleRap1 prevents fusions between long telomeres in fission yeasten_GB
dc.typeZeitschriftenaufsatzde
dc.identifier.doihttp://doi.org/10.25358/openscience-8694-
jgu.type.dinitypearticleen_GB
jgu.type.versionPublished versionde
jgu.type.resourceTextde
jgu.organisation.departmentFB 10 Biologiede
jgu.organisation.number7970-
jgu.organisation.nameJohannes Gutenberg-Universität Mainz-
jgu.rights.accessrightsopenAccess-
jgu.journal.titleThe EMBO journalde
jgu.journal.volume41de
jgu.journal.issue20de
jgu.pages.alternativee110458de
jgu.publisher.year2022-
jgu.publisher.nameWileyde
jgu.publisher.placeHoboken, NJ u.a.-
jgu.publisher.issn1460-2075de
jgu.organisation.placeMainz-
jgu.subject.ddccode570de
jgu.publisher.doi10.15252/embj.2021110458de
jgu.organisation.rorhttps://ror.org/023b0x485-
jgu.subject.dfgLebenswissenschaftende
Appears in collections:DFG-491381577-H

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