Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-8694
Authors: | Pan, Lili Tormey, Duncan Bobon, Nadine Baumann, Peter |
Title: | Rap1 prevents fusions between long telomeres in fission yeast |
Online publication date: | 30-Jan-2023 |
Year of first publication: | 2022 |
Language: | english |
Abstract: | The conserved Rap1 protein is part of the shelterin complex that plays critical roles in chromosome end protection and telomere length regulation. Previous studies have addressed how fission yeast Rap1 contributes to telomere length maintenance, but the mechanism by which the protein inhibits end fusions has remained elusive. Here, we use a mutagenesis screen in combination with high-throughput sequencing to identify several amino acid positions in Rap1 that have key roles in end protection. Interestingly, mutations at these sites render cells susceptible to genome instability in a conditional manner, whereby longer telomeres are prone to undergoing end fusions, while telomeres within the normal length range are sufficiently protected. The protection of long telomeres is in part dependent on their nuclear envelope attachment mediated by the Rap1–Bqt4 interaction. Our data demonstrate that long telomeres represent a challenge for the maintenance of genome integrity, thereby providing an explanation for species-specific upper limits on telomere length. |
DDC: | 570 Biowissenschaften 570 Life sciences |
Institution: | Johannes Gutenberg-Universität Mainz |
Department: | FB 10 Biologie |
Place: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-8694 |
Version: | Published version |
Publication type: | Zeitschriftenaufsatz |
License: | CC BY-NC-ND |
Information on rights of use: | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
Journal: | The EMBO journal 41 20 |
Pages or article number: | e110458 |
Publisher: | Wiley |
Publisher place: | Hoboken, NJ u.a. |
Issue date: | 2022 |
ISSN: | 1460-2075 |
Publisher DOI: | 10.15252/embj.2021110458 |
Appears in collections: | DFG-491381577-H |
Files in This Item:
File | Description | Size | Format | ||
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![]() | rap1_prevents_fusions_between-20230127154357329.pdf | 6.53 MB | Adobe PDF | View/Open |