Gutenberg Open Science: Tyramine actions on Drosophila flight behavior are affected by a glial dehydrogenase/reductase

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-8052

Full metadata record

DC Field	Value	Language
dc.contributor.author	Ryglewski, Stefanie	-
dc.contributor.author	Duch, Carsten	-
dc.contributor.author	Altenhein, Benjamin	-
dc.date.accessioned	2022-10-17T07:27:44Z	-
dc.date.available	2022-10-17T07:27:44Z	-
dc.date.issued	2017
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/8067	-
dc.description.abstract	The biogenic amines OA and TA modulate insect motor behavior in an antagonistic manner. OA generally enhances locomotor behaviors such as Drosophila larval crawling and flight, whereas TA decreases locomotor activity. However, the mechanisms and cellular targets of TA modulation of locomotor activity are incompletely understood. This study combines immunocytochemistry, genetics, and flight behavioral assays in the Drosophila model system to test the role of a candidate enzyme for TA catabolism, named nazgul (Naz), in flight motor behavioral control. We hypothesize that the dehydrogenase/reductases Naz represents a critical step in TA catabolism. Immunocytochemistry reveals that Naz is localized in a subset of Repo positive glial cells with cell bodies along the motor neuropil borders and numerous positive Naz arborizations extending into the synaptic flight motor neuropil. RNAi knock-down of Naz in Repo positive glial cells reduces Naz protein level below detection level by Western blotting. The resulting consequence is a reduction in flight durations, thus mimicking known motor behavioral phenotypes as resulting from increased TA levels. In accord with the interpretation that reduced TA degradation by Naz results in increased TA levels in the flight motor neuropil, the motor behavioral phenotype can be rescued by blocking TA receptors. Our findings indicate that TA modulates flight motor behavior by acting on central circuitry and that TA is normally taken up from the central motor neuropil by Repo-positive glial cells, desaminated, and further degraded by Naz.	en_GB
dc.description.sponsorship	DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin	de
dc.language.iso	eng	de
dc.rights	CC BY	*
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	*
dc.subject.ddc	570 Biowissenschaften	de_DE
dc.subject.ddc	570 Life sciences	en_GB
dc.title	Tyramine actions on Drosophila flight behavior are affected by a glial dehydrogenase/reductase	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-8052	-
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 10 Biologie	de
jgu.organisation.number	7970	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	Frontiers in systems neuroscience	de
jgu.journal.volume	11	de
jgu.pages.alternative	Art. 68	de
jgu.publisher.year	2017	-
jgu.publisher.name	Frontiers Research Foundation	de
jgu.publisher.place	Lausanne	de
jgu.publisher.uri	http://dx.doi.org/10.3389/fnsys.2017.00068	de
jgu.publisher.issn	1662-5137	de
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	570	de
opus.date.modified	2017-11-20T09:35:57Z
opus.subject.dfgcode	00-000
opus.organisation.string	FB 10: Biologie: Institut für Entwicklungsbiologie und Neurobiologie	de_DE
opus.identifier.opusid	57522
opus.institute.number	1012
opus.metadataonly	false
opus.type.contenttype	Keine	de_DE
opus.type.contenttype	None	en_EN
opus.affiliated	Ryglewski, Stefanie
opus.affiliated	Duch, Carsten
jgu.publisher.doi	10.3389/fnsys.2017.00068	de
jgu.organisation.ror	https://ror.org/023b0x485	-
Appears in collections:	DFG-OA-Publizieren (2012 - 2017)

Files in This Item:

	File	Description	Size	Format
	tyramine_actions_on_drosophil-20220925164356754.pdf		4.63 MB	Adobe PDF	View/Open

Show simple item record