Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-7996Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Shen, Limei | - |
| dc.contributor.author | Higuchi, Tetsuya | - |
| dc.contributor.author | Tubbe, Ingrid | - |
| dc.contributor.author | Voltz, Nicole | - |
| dc.contributor.author | Krummen, Mathias | - |
| dc.contributor.author | Pektor, Stefanie | - |
| dc.contributor.author | Montermann, Evelyn | - |
| dc.contributor.author | Rausch, Kristin | - |
| dc.contributor.author | Schmidt, Manfred | - |
| dc.contributor.author | Schild, Hansjörg | - |
| dc.contributor.author | Grabbe, Stephan | - |
| dc.contributor.author | Bros, Matthias | - |
| dc.date.accessioned | 2022-10-14T07:10:12Z | - |
| dc.date.available | 2022-10-14T07:10:12Z | - |
| dc.date.issued | 2013 | - |
| dc.identifier.uri | https://openscience.ub.uni-mainz.de/handle/20.500.12030/8011 | - |
| dc.description.abstract | Dendritic cells (DCs) constitute an attractive target for specific delivery of nanovaccines for immunotherapeutic applications. Here we tested nano-sized dextran (DEX) particles to serve as a DC-addressing nanocarrier platform. Non-functionalized DEX particles had no immunomodulatory effect on bone marrow (BM)-derived murine DCs in vitro. However, when adsorbed with ovalbumine (OVA), DEX particles were efficiently engulfed by BM-DCs in a mannose receptor-dependent manner. A DEX-based nanovaccine containing OVA and lipopolysaccharide (LPS) as a DC stimulus induced strong OVA peptide-specific CD4(+) and CD8(+) T cell proliferation both in vitro and upon systemic application in mice, as well as a robust OVA-specific humoral immune response (IgG1>IgG2a) in vivo. Accordingly, this nanovaccine also raised both a more pronounced delayed-type hypersensitivity response and a stronger induction of cytotoxic CD8(+) T cells than obtained upon administration of OVA and LPS in soluble form. Therefore, DEX-based nanoparticles constitute a potent, versatile and easy to prepare nanovaccine platform for immunotherapeutic approaches. | en_GB |
| dc.description.sponsorship | DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin | de |
| dc.language.iso | eng | de |
| dc.rights | CC BY | * |
| dc.rights.uri | https://creativecommons.org/licenses/by/3.0/ | * |
| dc.subject.ddc | 610 Medizin | de_DE |
| dc.subject.ddc | 610 Medical sciences | en_GB |
| dc.title | A trifunctional dextran-based nanovaccine targets and activates murine dendritic cells, and induces potent cellular and humoral immune responses in vivo | en_GB |
| dc.type | Zeitschriftenaufsatz | de |
| dc.identifier.doi | http://doi.org/10.25358/openscience-7996 | - |
| jgu.type.dinitype | article | en_GB |
| jgu.type.version | Published version | de |
| jgu.type.resource | Text | de |
| jgu.organisation.department | FB 04 Medizin | de |
| jgu.organisation.department | FB 09 Chemie, Pharmazie u. Geowissensch. | de |
| jgu.organisation.number | 2700 | - |
| jgu.organisation.number | 7950 | - |
| jgu.organisation.name | Johannes Gutenberg-Universität Mainz | - |
| jgu.rights.accessrights | openAccess | - |
| jgu.journal.title | PLoS one | de |
| jgu.journal.volume | 8 | de |
| jgu.journal.issue | 12 | de |
| jgu.pages.alternative | e80904 | de |
| jgu.publisher.year | 2013 | - |
| jgu.publisher.name | PLoS | de |
| jgu.publisher.place | Lawrence, Kan. | de |
| jgu.publisher.uri | http://dx.doi.org/10.1371/journal.pone.0080904 | de |
| jgu.publisher.issn | 1932-6203 | de |
| jgu.organisation.place | Mainz | - |
| jgu.identifier.pmid | 24339889 | - |
| jgu.subject.ddccode | 610 | de |
| opus.date.modified | 2018-08-08T09:09:52Z | - |
| opus.subject.dfgcode | 00-000 | - |
| opus.organisation.string | FB 04: Medizin: Institut für Immunologie | de_DE |
| opus.organisation.string | FB 04: Medizin: Klinik und Poliklinik für Nuklearmedizin | de_DE |
| opus.organisation.string | FB 04: Medizin: Hautklinik | de_DE |
| opus.organisation.string | FB 09: Chemie, Pharmazie und Geowissenschaften: Institut für Physikalische Chemie | de_DE |
| opus.identifier.opusid | 27386 | - |
| opus.importsource | pubmed | - |
| opus.institute.number | 0412 | - |
| opus.institute.number | 0422 | - |
| opus.institute.number | 0431 | - |
| opus.institute.number | 0906 | - |
| opus.metadataonly | false | - |
| opus.type.contenttype | Keine | de_DE |
| opus.type.contenttype | None | en_EN |
| opus.affiliated | Pektor, Stefanie | - |
| opus.affiliated | Schmidt, Manfred | - |
| opus.affiliated | Schild, Hansjörg | - |
| opus.affiliated | Grabbe, Stephan | - |
| opus.affiliated | Bros, Matthias | - |
| jgu.publisher.doi | 10.1371/journal.pone.0080904 | de |
| jgu.organisation.ror | https://ror.org/023b0x485 | - |
| Appears in collections: | DFG-OA-Publizieren (2012 - 2017) | |
Files in This Item:
| File | Description | Size | Format | ||
|---|---|---|---|---|---|
 | a_trifunctional_dextranbased_-20220925162029392.pdf | 1.73 MB | Adobe PDF | View/Open |