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Authors: Perumal, Natarajan
Manicam, Caroline
Steinicke, Matthias
Funke, Sebastian
Pfeiffer, Norbert
Grus, Franz-Hermann
Title: Characterization of the human aqueous humour proteome : a comparison of the genders
Online publication date: 13-Oct-2022
Year of first publication: 2017
Language: english
Abstract: Aqueous humour (AH) is an important biologic fluid that maintains normal intraocular pressure and contains proteins that regulate the homeostasis of ocular tissues. Any alterations in the protein compositions are correlated to the pathogenesis of various ocular disorders. In recent years, gender-based medicine has emerged as an important research focus considering the prevalence of certain diseases, which are higher in a particular sex. Nevertheless, the inter-gender variations in the AH proteome are unknown. Therefore, this study endeavoured to characterize the AH proteome to assess the differences between genders. Thirty AH samples of patients who underwent cataract surgery were categorized according to their gender. Label-free quantitative discovery mass spectrometry-based proteomics strategy was employed to characterize the AH proteome. A total of 147 proteins were identified with a false discovery rate of less than 1% and only the top 10 major AH proteins make up almost 90% of the total identified proteins. A large number of proteins identified were correlated to defence, immune and inflammatory mechanisms, and response to wounding. Four proteins were found to be differentially abundant between the genders, comprising SERPINF1, SERPINA3, SERPING1 and PTGDS. The findings emerging from our study provide the first insight into the gender-based proteome differences in the AH and also highlight the importance in considering potential sex-dependent changes in the proteome of ocular pathologies in future studies employing the AH.
DDC: 610 Medizin
610 Medical sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 04 Medizin
Place: Mainz
Version: Published version
Publication type: Zeitschriftenaufsatz
License: CC BY
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Journal: PLOS ONE
Pages or article number: e0172481
Publisher: PLOS
Publisher place: San Francisco, California, US
Issue date: 2017
ISSN: 1932-6203
Publisher URL:
Publisher DOI: 10.1371/journal.pone.0172481
Appears in collections:DFG-OA-Publizieren (2012 - 2017)

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