Autoren:	Schmidt, Hanno Greshake, Bastian Feldmeyer, Barbara Hankeln, Thomas Pfenninger, Markus
Titel:	Genomic basis of ecological niche divergence among cryptic sister species of non-biting midges
Online-Publikationsdatum:	13-Okt-2022
Erscheinungsdatum:	2013
Sprache des Dokuments:	Englisch
Zusammenfassung/Abstract:	BACKGROUND There is a lack of understanding the evolutionary forces driving niche segregation of closely related organisms. In addition, pinpointing the genes driving ecological divergence is a key goal in molecular ecology. Here, larval transcriptome sequences obtained by next-generation-sequencing are used to address these issues in a morphologically cryptic sister species pair of non-biting midges (Chironomus riparius and C. piger). RESULTS More than eight thousand orthologous open reading frames were screened for interspecific divergence and intraspecific polymorphisms. Despite a small mean sequence divergence of 1.53% between the sister species, 25.1% of 18,115 observed amino acid substitutions were inferred by α statistics to be driven by positive selection. Applying McDonald-Kreitman tests to 715 alignments of gene orthologues identified eleven (1.5%) genes driven by positive selection. CONCLUSIONS Three candidate genes were identified as potentially responsible for the observed niche segregation concerning nitrite concentration, habitat temperature and water conductivity. Additionally, signs of positive selection in the hydrogen sulfide detoxification pathway were detected, providing a new plausible hypothesis for the species' ecological differentiation. Finally, a divergently selected, nuclear encoded mitochondrial ribosomal protein may contribute to reproductive isolation due to cytonuclear coevolution.
DDC-Sachgruppe:	570 Biowissenschaften 570 Life sciences
Veröffentlichende Institution:	Johannes Gutenberg-Universität Mainz
Organisationseinheit:	FB 10 Biologie
Veröffentlichungsort:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-7974
Version:	Published version
Publikationstyp:	Zeitschriftenaufsatz
Nutzungsrechte:	CC BY
Informationen zu den Nutzungsrechten:	https://creativecommons.org/licenses/by/2.0/
Zeitschrift:	BMC genomics 14
Seitenzahl oder Artikelnummer:	Art. 384
Verlag:	BioMed Central
Verlagsort:	London
Erscheinungsdatum:	2013
ISSN:	1471-2164
URL der Originalveröffentlichung:	http://dx.doi.org/10.1186/1471-2164-14-384
DOI der Originalveröffentlichung:	10.1186/1471-2164-14-384
Enthalten in den Sammlungen:	DFG-OA-Publizieren (2012 - 2017)