Gutenberg Open Science: Cytotoxicity and modes of action of the methanol extracts of six Cameroonian medicinal plants against multidrug-resistant tumor cells

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-7885

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DC Field	Value	Language
dc.contributor.author	Kuete, Victor	-
dc.contributor.author	Fankam, Aimé G.	-
dc.contributor.author	Wiench, Benjamin	-
dc.contributor.author	Efferth, Thomas	-
dc.date.accessioned	2022-10-06T07:51:12Z	-
dc.date.available	2022-10-06T07:51:12Z	-
dc.date.issued	2013	-
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/7900	-
dc.description.abstract	Introduction. The present study aims at evaluating the cytotoxicity of twelve parts from six Cameroonian medicinal plants on sensitive and drug-resistant cancer cell lines. We also studied the mode of action of the most active plants, Gladiolus quartinianus, Vepris soyauxii, and Anonidium mannii. Methods. The cytotoxicity of the extracts was determined using a resazurin assay. Flow cytometry was used for cell-cycle analysis and detection of apoptosis, analysis of mitochondrial membrane potential (MMP), and measurement of reactive oxygen species (ROS). Results. At 40 g/mL, three extracts showed a growth of CCRF-CEM leukemia cells by less than 50%. This includes the extracts from G. quartinianus (GQW; 25.69%), Vepris soyauxii leaves (VSL; 29.82%), and Anonidium mannii leaves (AML; 31.58%). The lowest IC50 values below 30 mu g/mL were obtained with GQW, AML and VSL against 7/9, 8/9, and 9/9 tested cancer cell lines, respectively. The lowest IC50 values for each plant were 4.09 mu g/mL, and 9.14 mu g/mL (against U87MG.DeltaEGFR cells), respectively, for VSL and AML and 10.57 mu g/mL (against CCRF-CEM cells) for GQW. GQW induced cell cycle arrest between G0/G1 and S phases, whilst VSL and AML induced arrest in G0/G1. All three extracts induced apoptosis in CCRF-CEM cells by loss of MMP, whilst AML also enhanced production of ROS. Conclusion. The three active plants may be a source for the development of new anticancer drugs.	en_GB
dc.description.sponsorship	DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin	de
dc.language.iso	eng	de
dc.rights	CC BY	*
dc.rights.uri	https://creativecommons.org/licenses/by/3.0/	*
dc.subject.ddc	570 Biowissenschaften	de_DE
dc.subject.ddc	570 Life sciences	en_GB
dc.title	Cytotoxicity and modes of action of the methanol extracts of six Cameroonian medicinal plants against multidrug-resistant tumor cells	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-7885	-
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 09 Chemie, Pharmazie u. Geowissensch.	de
jgu.organisation.number	7950	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	Evidence-based complementary and alternative medicine	de
jgu.journal.volume	2013	de
jgu.pages.alternative	Art. 285903	de
jgu.publisher.year	2013	-
jgu.publisher.name	Hindawi	de
jgu.publisher.place	New York, NY	de
jgu.publisher.uri	http://dx.doi.org/10.1155/2013/285903	de
jgu.publisher.issn	1741-4288	de
jgu.publisher.issn	1741-427X	de
jgu.organisation.place	Mainz	-
jgu.identifier.pmid	24174974	-
jgu.subject.ddccode	570	de
opus.date.modified	2018-07-31T10:06:33Z	-
opus.subject.dfgcode	00-000	-
opus.organisation.string	FB 09: Chemie, Pharmazie und Geowissenschaften: Institut für Pharmazie	de_DE
opus.identifier.opusid	24660	-
opus.importsource	pubmed	-
opus.institute.number	0908	-
opus.metadataonly	false	-
opus.type.contenttype	Keine	de_DE
opus.type.contenttype	None	en_EN
opus.affiliated	Efferth, Thomas	-
jgu.publisher.doi	10.1155/2013/285903	de
jgu.organisation.ror	https://ror.org/023b0x485	-
Appears in collections:	DFG-OA-Publizieren (2012 - 2017)

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