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Authors: Reichel, Christoph A.
Hessenauer, Maximilian E. T.
Pflieger, Kerstin
Rehberg, Markus
Kanse, Sandip M.
Zahler, Stefan
Krombach, Fritz
Berghaus, Alexander
Strieth, Sebastian
Title: Components of the plasminogen activation system promote engraftment of porous polyethylene biomaterial via common and distinct effects
Online publication date: 5-Oct-2022
Year of first publication: 2015
Language: english
Abstract: Rapid fibrovascularization is a prerequisite for successful biomaterial engraftment. In addition to their well-known roles in fibrinolysis, urokinase-type plasminogen activator (uPA) and tissue plasminogen activator (tPA) or their inhibitor plasminogen activator inhibitor-1 (PAI-1) have recently been implicated as individual mediators in non-fibrinolytic processes, including cell adhesion, migration, and proliferation. Since these events are critical for fibrovascularization of biomaterial, we hypothesized that the components of the plasminogen activation system contribute to biomaterial engraftment. Employing in vivo and ex vivo microscopy techniques, vessel and collagen network formation within porous polyethylene (PPE) implants engrafted into dorsal skinfold chambers were found to be significantly impaired in uPA-, tPA-, or PAI-1-deficient mice. Consequently, the force required for mechanical disintegration of the implants out of the host tissue was significantly lower in the mutant mice than in wild-type controls. Conversely, surface coating with recombinant uPA, tPA, non-catalytic uPA, or PAI-1, but not with non-catalytic tPA, accelerated implant vascularization in wild-type mice. Thus, uPA, tPA, and PAI-1 contribute to the fibrovascularization of PPE implants through common and distinct effects. As clinical perspective, surface coating with recombinant uPA, tPA, or PAI-1 might provide a novel strategy for accelerating the vascularization of this biomaterial.
DDC: 610 Medizin
610 Medical sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 04 Medizin
Place: Mainz
Version: Published version
Publication type: Zeitschriftenaufsatz
License: CC BY
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Journal: PLoS one
Pages or article number: e0116883
Publisher: PLoS
Publisher place: Lawrence, Kan.
Issue date: 2015
ISSN: 1932-6203
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Publisher DOI: 10.1371/journal.pone.0116883
Appears in collections:DFG-OA-Publizieren (2012 - 2017)

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