Gutenberg Open Science: Noncanonical expression of a murine cytomegalovirus early protein CD8 T-cell epitope as an immediate early epitope based on transcription from an upstream gene

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-7807

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DC Field	Value	Language
dc.contributor.author	Fink, Annette	-
dc.contributor.author	Büttner, Julia K.	-
dc.contributor.author	Thomas, Doris	-
dc.contributor.author	Holtappels, Rafaela	-
dc.contributor.author	Reddehase, Matthias J.	-
dc.contributor.author	Lemmermann, Niels A. W.	-
dc.date.accessioned	2022-10-04T09:30:37Z	-
dc.date.available	2022-10-04T09:30:37Z	-
dc.date.issued	2014	-
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/7822	-
dc.description.abstract	Viral CD8 T-cell epitopes, represented by viral peptides bound to major histocompatibility complex class-I (MHC-I) glycoproteins, are often identified by "reverse immunology", a strategy not requiring biochemical and structural knowledge of the actual viral protein from which they are derived by antigen processing. Instead, bioinformatic algorithms predicting the probability of C-terminal cleavage in the proteasome, as well as binding affinity to the presenting MHC-I molecules, are applied to amino acid sequences deduced from predicted open reading frames (ORFs) based on the genomic sequence. If the protein corresponding to an antigenic ORF is known, it is usually inferred that the kinetic class of the protein also defines the phase in the viral replicative cycle during which the respective antigenic peptide is presented for recognition by CD8 T cells. We have previously identified a nonapeptide from the predicted ORFm164 of murine cytomegalovirus that is presented by the MHC-I allomorph H-2 Dd and that is immunodominant in BALB/c (H-2d haplotype) mice. Surprisingly, although the ORFm164 protein gp36.5 is expressed as an Early (E) phase protein, the m164 epitope is presented already during the Immediate Early (IE) phase, based on the expression of an upstream mRNA starting within ORFm167 and encompassing ORFm164.	en_GB
dc.description.sponsorship	DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin	de
dc.language.iso	eng	de
dc.rights	CC BY	*
dc.rights.uri	https://creativecommons.org/licenses/by/3.0/	*
dc.subject.ddc	610 Medizin	de_DE
dc.subject.ddc	610 Medical sciences	en_GB
dc.title	Noncanonical expression of a murine cytomegalovirus early protein CD8 T-cell epitope as an immediate early epitope based on transcription from an upstream gene	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-7807	-
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 04 Medizin	de
jgu.organisation.number	2700	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	Viruses	de
jgu.journal.volume	6	de
jgu.journal.issue	2	de
jgu.pages.start	808	de
jgu.pages.end	831	de
jgu.publisher.year	2014	-
jgu.publisher.name	MDPI	de
jgu.publisher.place	Basel	de
jgu.publisher.uri	http://dx.doi.org/10.3390/v6020808	de
jgu.publisher.issn	1999-4915	de
jgu.organisation.place	Mainz	-
jgu.identifier.pmid	24535000	-
jgu.subject.ddccode	610	de
opus.date.modified	2018-08-08T08:17:34Z	-
opus.subject.dfgcode	00-000	-
opus.organisation.string	FB 04: Medizin: Institut für Virologie	de_DE
opus.identifier.opusid	27327	-
opus.importsource	pubmed	-
opus.institute.number	0409	-
opus.metadataonly	false	-
opus.type.contenttype	Keine	de_DE
opus.type.contenttype	None	en_EN
opus.affiliated	Holtappels, Rafaela	-
opus.affiliated	Reddehase, Matthias J.	-
jgu.publisher.doi	10.3390/v6020808	de
jgu.organisation.ror	https://ror.org/023b0x485	-
Appears in collections:	DFG-OA-Publizieren (2012 - 2017)

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