Authors:	Manicam, Caroline Perumal, Natarajan Pfeiffer, Norbert Grus, Franz-Hermann Gericke, Adrian
Title:	First insight into the proteome landscape of the porcine short posterior ciliary arteries : key signalling pathways maintaining physiologic functions
Online publication date:	4-Oct-2022
Year of first publication:	2016
Language:	english
Abstract:	Short posterior ciliary arteries (sPCA) provide the major blood supply to the optic nerve head. Emerging evidence has linked structural and functional anomalies of sPCA to the pathogenesis of several ocular disorders that cause varying degrees of visual loss, particularly anterior ischaemic optic neuropathy and glaucoma. Although the functional relevance of this vascular bed is well-recognized, the proteome of sPCA remains uncharacterized. Since the porcine ocular system closely resembles that of the human’s and is increasingly employed in translational ophthalmic research, this study characterized the proteome of porcine sPCA employing the mass spectrometry-based proteomics strategy. A total of 1742 proteins and 10527 peptides were identified in the porcine sPCA. The major biological processes involved in the maintenance of physiological functions of the sPCA included redox and metabolic processes, and cytoskeleton organization. These proteins were further clustered into diverse signalling pathways that regulate vasoactivity of sPCA, namely the tight junction, α- and β-adrenoceptor, 14-3-3, nitric oxide synthase and endothelin-1 -mediated signalling pathways. This study provides the first insight into the complex mechanisms dictating the vast protein repertoire in normal vascular physiology of the porcine sPCA. It is envisioned that our findings will serve as important benchmarks for future studies of sPCA.
DDC:	610 Medizin 610 Medical sciences
Institution:	Johannes Gutenberg-Universität Mainz
Department:	FB 04 Medizin
Place:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-7804
Version:	Published version
Publication type:	Zeitschriftenaufsatz
License:	CC BY
Information on rights of use:	https://creativecommons.org/licenses/by/4.0/
Journal:	Scientific reports 6
Pages or article number:	Art. 8298
Publisher:	Nature Publishing Group
Publisher place:	London
Issue date:	2016
ISSN:	2045-2322
Publisher URL:	http://dx.doi.org/10.1038/srep38298
Publisher DOI:	10.1038/srep38298
Appears in collections:	DFG-OA-Publizieren (2012 - 2017)