Gutenberg Open Science: Plasmacytoid dendritic cells are inefficient in activation of human regulatory T cells

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-7799

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DC Field	Value	Language
dc.contributor.author	Hubo, Mario	-
dc.contributor.author	Jonuleit, Helmut	-
dc.date.accessioned	2022-10-04T08:35:06Z	-
dc.date.available	2022-10-04T08:35:06Z	-
dc.date.issued	2012	-
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/7814	-
dc.description.abstract	BACKGROUND: Dendritic cells (DC) play a key role in initiation and regulation of immune responses. Plasmacytoid DC (pDC), a small subset of DC, characterized as type-I interferon producing cells, are critically involved in anti-viral immune responses, but also mediate tolerance by induction of regulatory T cells (Treg). In this study, we compared the capacity of human pDC and conventional DC (cDC) to modulate T cell activity in presence of Foxp3(+) Treg. PRINCIPAL FINDINGS: In coculture of T effector cells (Teff) and Treg, activated cDC overcome Treg anergy, abrogate their suppressive function and induce Teff proliferation. In contrast, pDC do not break Treg anergy but induce Teff proliferation even in coculture with Treg. Lack of Treg-mediated suppression is independent of proinflammatory cytokines like IFN-alpha, IL-1, IL-6 and TNF-alpha. Phenotyping of pDC-stimulated Treg reveals a reduced expression of Treg activation markers GARP and CTLA-4. Additional stimulation by anti-CD3 antibodies enhances surface expression of GARP and CTLA-4 on Treg and consequently reconstitutes their suppressive function, while increased costimulation with anti-CD28 antibodies is ineffective. CONCLUSIONS/SIGNIFICANCE: Our data show that activated pDC induce Teff proliferation, but are insufficient for functional Treg activation and, therefore, allow expansion of Teff also in presence of Treg.	en_GB
dc.description.sponsorship	DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin	de
dc.language.iso	eng	de
dc.rights	CC BY	*
dc.rights.uri	https://creativecommons.org/licenses/by/3.0/	*
dc.subject.ddc	610 Medizin	de_DE
dc.subject.ddc	610 Medical sciences	en_GB
dc.title	Plasmacytoid dendritic cells are inefficient in activation of human regulatory T cells	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-7799	-
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 04 Medizin	de
jgu.organisation.number	2700	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	PLoS one	de
jgu.journal.volume	7	de
jgu.journal.issue	8	de
jgu.pages.alternative	e44056	de
jgu.publisher.year	2012	-
jgu.publisher.name	PLoS	de
jgu.publisher.place	Lawrence, Kan.	de
jgu.publisher.uri	http://dx.doi.org/10.1371/journal.pone.0044056	de
jgu.publisher.issn	1932-6203	de
jgu.organisation.place	Mainz	-
jgu.identifier.pmid	992pubmed	-
jgu.subject.ddccode	610	de
opus.date.modified	2013-07-02T08:40:06Z	-
opus.subject.dfgcode	04-204	-
opus.organisation.string	FB 04: Medizin: Hautklinik	de_DE
opus.identifier.opusid	22647	-
opus.importsource	pubmed	-
opus.institute.number	0431	-
opus.metadataonly	false	-
opus.type.contenttype	Forschungsbericht	de_DE
opus.type.contenttype	Research Report	en_EN
opus.affiliated	Jonuleit, Helmut	-
jgu.publisher.doi	10.1371/journal.pone.0044056	de
jgu.organisation.ror	https://ror.org/023b0x485	-
Appears in collections:	DFG-OA-Publizieren (2012 - 2017)

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