Gutenberg Open Science: Glaucoma related proteomic alterations in human retina samples

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-7433

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DC Field	Value	Language
dc.contributor.author	Funke, Sebastian	-
dc.contributor.author	Perumal, Natarajan	-
dc.contributor.author	Beck, Sabine	-
dc.contributor.author	Gabel-Scheurich, Silke	-
dc.contributor.author	Schmelter, Carsten	-
dc.contributor.author	Teister, Julia	-
dc.contributor.author	Gerbig, Claudia	-
dc.contributor.author	Gramlich, Oliver W.	-
dc.contributor.author	Pfeiffer, Norbert	-
dc.contributor.author	Grus, Franz-Hermann	-
dc.date.accessioned	2022-07-15T09:39:42Z	-
dc.date.available	2022-07-15T09:39:42Z	-
dc.date.issued	2016
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/7447	-
dc.description.abstract	Glaucoma related proteomic changes have been documented in cell and animal models. However, proteomic studies investigating on human retina samples are still rare. In the present work, retina samples of glaucoma and non-glaucoma control donors have been examined by a state-of-the-art mass spectrometry (MS) workflow to uncover glaucoma related proteomic changes. More than 600 proteins could be identified with high confidence (FDR < 1%) in human retina samples. Distinct proteomic changes have been observed in 10% of proteins encircling mitochondrial and nucleus species. Numerous proteins showed a significant glaucoma related level change (p < 0.05) or distinct tendency of alteration (p < 0.1). Candidates were documented to be involved in cellular development, stress and cell death. Increase of stress related proteins and decrease of new glaucoma related candidates, ADP/ATP translocase 3 (ANT3), PC4 and SRFS1-interacting protein 1 (DFS70) and methyl-CpG-binding protein 2 (MeCp2) could be documented by MS. Moreover, candidates could be validated by Accurate Inclusion Mass Screening (AIMS) and immunostaining and supported for the retinal ganglion cell layer (GCL) by laser capture microdissection (LCM) in porcine and human eye cryosections. The workflow allowed a detailed view into the human retina proteome highlighting new molecular players ANT3, DFS70 and MeCp2 associated to glaucoma.	en_GB
dc.description.sponsorship	DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin	de
dc.language.iso	eng	de
dc.rights	CC BY	*
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	*
dc.subject.ddc	610 Medizin	de_DE
dc.subject.ddc	610 Medical sciences	en_GB
dc.title	Glaucoma related proteomic alterations in human retina samples	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-7433	-
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 04 Medizin	de
jgu.organisation.number	2700	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	Scientific reports	de
jgu.journal.volume	6	de
jgu.pages.alternative	Art. 29759	de
jgu.publisher.year	2016	-
jgu.publisher.name	Nature Publishing Group	de
jgu.publisher.place	London	de
jgu.publisher.uri	http://dx.doi.org/10.1038/srep29759	de
jgu.publisher.issn	2045-2322	de
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	610	de
opus.date.modified	2018-08-23T08:47:34Z
opus.subject.dfgcode	00-000
opus.organisation.string	FB 04: Medizin: Augenklinik und Poliklinik	de_DE
opus.identifier.opusid	56390
opus.institute.number	0446
opus.metadataonly	false
opus.type.contenttype	Keine	de_DE
opus.type.contenttype	None	en_EN
opus.affiliated	Pfeiffer, Norbert
opus.affiliated	Grus, Franz-Hermann
jgu.publisher.doi	10.1038/srep29759	de
jgu.organisation.ror	https://ror.org/023b0x485	-
Appears in collections:	DFG-OA-Publizieren (2012 - 2017)

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