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Authors: Funke, Sebastian
Perumal, Natarajan
Beck, Sabine
Gabel-Scheurich, Silke
Schmelter, Carsten
Teister, Julia
Gerbig, Claudia
Gramlich, Oliver W.
Pfeiffer, Norbert
Grus, Franz-Hermann
Title: Glaucoma related proteomic alterations in human retina samples
Online publication date: 15-Jul-2022
Year of first publication: 2016
Language: english
Abstract: Glaucoma related proteomic changes have been documented in cell and animal models. However, proteomic studies investigating on human retina samples are still rare. In the present work, retina samples of glaucoma and non-glaucoma control donors have been examined by a state-of-the-art mass spectrometry (MS) workflow to uncover glaucoma related proteomic changes. More than 600 proteins could be identified with high confidence (FDR < 1%) in human retina samples. Distinct proteomic changes have been observed in 10% of proteins encircling mitochondrial and nucleus species. Numerous proteins showed a significant glaucoma related level change (p < 0.05) or distinct tendency of alteration (p < 0.1). Candidates were documented to be involved in cellular development, stress and cell death. Increase of stress related proteins and decrease of new glaucoma related candidates, ADP/ATP translocase 3 (ANT3), PC4 and SRFS1-interacting protein 1 (DFS70) and methyl-CpG-binding protein 2 (MeCp2) could be documented by MS. Moreover, candidates could be validated by Accurate Inclusion Mass Screening (AIMS) and immunostaining and supported for the retinal ganglion cell layer (GCL) by laser capture microdissection (LCM) in porcine and human eye cryosections. The workflow allowed a detailed view into the human retina proteome highlighting new molecular players ANT3, DFS70 and MeCp2 associated to glaucoma.
DDC: 610 Medizin
610 Medical sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 04 Medizin
Place: Mainz
Version: Published version
Publication type: Zeitschriftenaufsatz
License: CC BY
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Journal: Scientific reports
Pages or article number: Art. 29759
Publisher: Nature Publishing Group
Publisher place: London
Issue date: 2016
ISSN: 2045-2322
Publisher URL:
Publisher DOI: 10.1038/srep29759
Appears in collections:DFG-OA-Publizieren (2012 - 2017)

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