Gutenberg Open Science: Hypoxia impairs agonist-induced integrin αIIbβ3 activation and platelet aggregation

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-7417

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DC Field	Value	Language
dc.contributor.author	Kiouptsi, Klytaimnistra	-
dc.contributor.author	Gambaryan, Stepan	-
dc.contributor.author	Walter, Elena	-
dc.contributor.author	Walter, Ulrich	-
dc.contributor.author	Jurk, Kerstin	-
dc.contributor.author	Reinhardt, Christoph	-
dc.date.accessioned	2022-07-14T08:24:34Z	-
dc.date.available	2022-07-14T08:24:34Z	-
dc.date.issued	2017
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/7431	-
dc.description.abstract	Under ischemic conditions, tissues are exposed to hypoxia. Although human physiology, to a certain extent, can adapt to hypoxic conditions, the impact of low oxygen levels on platelet function is unresolved. Therefore, we explored how reduction of atmospheric oxygen levels to 1% might affect agonist-induced aggregation and static adhesion of isolated human platelets. We uncovered that isolated, washed human platelets exposed to hypoxic conditions show reduced thrombin receptor-activating peptide-6 (TRAP-6) and convulxin-induced aggregation. Of note, this hypoxia-triggered effect was not observed in platelet-rich plasma. Independent of the agonist used (TRAP-6, ADP), activation of the platelet fibrinogen receptor integrin αIIbβ3 (GPIIbIIIa, CD41/CD61) was strongly reduced at 1% and 8% oxygen. The difference in agonist-induced integrin αIIbβ3 activation was apparent within 5 minutes of stimulation. Following hypoxia, re-oxygenation resulted in the recovery of integrin αIIbβ3 activation. Importantly, platelet secretion was not impaired by hypoxia. Static adhesion experiments revealed decreased platelet deposition to fibrinogen coatings, but not to collagen or vitronectin coatings, indicating that specifically the function of the integrin subunit αIIb is impaired by exposure of platelets to reduced oxygen levels. Our results reveal an unexpected effect of oxygen deprivation on platelet aggregation mediated by the fibrinogen receptor integrin αIIbβ3.	en_GB
dc.description.sponsorship	DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin	de
dc.language.iso	eng	de
dc.rights	CC BY	*
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	*
dc.subject.ddc	610 Medizin	de_DE
dc.subject.ddc	610 Medical sciences	en_GB
dc.title	Hypoxia impairs agonist-induced integrin αIIbβ3 activation and platelet aggregation	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-7417	-
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 04 Medizin	de
jgu.organisation.number	2700	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	Scientific reports	de
jgu.journal.volume	7	de
jgu.pages.alternative	Art. 7621	de
jgu.publisher.year	2017	-
jgu.publisher.name	Macmillan Publishers Limited, part of Springer Nature	de
jgu.publisher.place	London	de
jgu.publisher.uri	http://dx.doi.org/10.1038/s41598-017-07988-x	de
jgu.publisher.issn	2045-2322	de
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	610	de
opus.date.modified	2019-01-22T10:33:59Z
opus.subject.dfgcode	00-000
opus.organisation.string	FB 04: Medizin: Centrum für Thrombose und Hämostase (CTH)	de_DE
opus.identifier.opusid	57990
opus.institute.number	0463
opus.metadataonly	false
opus.type.contenttype	Keine	de_DE
opus.type.contenttype	None	en_EN
opus.affiliated	Walter, Ulrich
opus.affiliated	Reinhardt, Christoph
jgu.publisher.doi	10.1038/s41598-017-07988-x	de
jgu.organisation.ror	https://ror.org/023b0x485	-
Appears in collections:	DFG-OA-Publizieren (2012 - 2017)

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