Gutenberg Open Science: Distribution of cytoglobin in the mouse brain

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-7397

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DC Field	Value	Language
dc.contributor.author	Reuss, Stefan	-
dc.contributor.author	Wystub, Sylvia	-
dc.contributor.author	Disque-Kaiser, Ursula	-
dc.contributor.author	Hankeln, Thomas	-
dc.contributor.author	Burmester, Thorsten	-
dc.date.accessioned	2022-07-13T09:58:51Z	-
dc.date.available	2022-07-13T09:58:51Z	-
dc.date.issued	2016
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/7411	-
dc.description.abstract	Cytoglobin (Cygb) is a vertebrate globin with so far poorly defined function. It is expressed in the fibroblast cell-lineage but has also been found in neurons. Here we provide, using immunohistochemistry, a detailed study on the distribution of Cygb in the mouse brain. While Cygb is a cytoplasmic protein in active cells of the supportive tissue, in neurons it is located in the cytoplasm and the nucleus. We found the expression of Cygb in all brain regions, although only a fraction of the neurons was Cygb-positive. Signals were of different intensity ranging from faint to very intense. Telencephalic neurons in all laminae of the cerebral cortex (CCo), in the olfactory bulb (in particular periglomerular cells), in the hippocampal formation (strongly stained pyramidal cells with long processes), basal ganglia (scattered multipolar neurons in the dorsal striatum, dorsal and ventral pallidum (VP)), and in the amygdala (neurons with unlabeled processes) were labeled by the antibody. In the diencephalon, we observed Cygb-positive neurons of moderate intensity in various nuclei of the dorsal thalamus, in the hypothalamus, metathalamus (geniculate nuclei), epithalamus with strong labeling of habenular nucleus neurons and no labeling of pineal cells, and in the ventral thalamus. Tegmental neurons stood out by strongly stained somata with long processes in, e.g., the laterodorsal nucleus. In the tectum, faintly labeled neurons and fibers were detected in the superior colliculus (SC). The cerebellum exhibited unlabeled Purkinje-neurons but signs of strong afferent cortical innervation. Neurons in the gray matter of the spinal cord showed moderate immunofluorescence. Peripheral ganglia were not labeled by the antibody. The Meynert-fascicle and the olfactory and optic nerves/tracts were the only Cygb-immunoreactive (Cygb-IR) fiber systems. Notably, we found a remarkable level of colocalization of Cygb and neuronal nitric oxide (NO)-synthase in neurons, which supports a functional association.	en_GB
dc.description.sponsorship	DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin	de
dc.language.iso	eng	de
dc.rights	CC BY	*
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	*
dc.subject.ddc	610 Medizin	de_DE
dc.subject.ddc	610 Medical sciences	en_GB
dc.title	Distribution of cytoglobin in the mouse brain	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-7397	-
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 04 Medizin	de
jgu.organisation.department	FB 10 Biologie	de
jgu.organisation.number	2700	-
jgu.organisation.number	7970	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	Frontiers in neuroanatomy	de
jgu.journal.volume	10	de
jgu.pages.alternative	Art. 47	de
jgu.publisher.year	2016	-
jgu.publisher.name	Frontiers Research Foundation	de
jgu.publisher.place	Lausanne	de
jgu.publisher.uri	http://dx.doi.org/10.3389/fnana.2016.00047	de
jgu.publisher.issn	1662-5129	de
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	610	de
opus.date.modified	2018-08-23T08:16:56Z
opus.subject.dfgcode	00-000
opus.organisation.string	FB 04: Medizin: Klinik und Poliklinik für Nuklearmedizin	de_DE
opus.organisation.string	FB 10: Biologie: Institut für Molekulargenetik, Gentechnologische Sicherheitsforschung und Beratung	de_DE
opus.identifier.opusid	56309
opus.institute.number	0422
opus.institute.number	1006
opus.metadataonly	false
opus.type.contenttype	Keine	de_DE
opus.type.contenttype	None	en_EN
opus.affiliated	Reuss, Stefan
opus.affiliated	Hankeln, Thomas
jgu.publisher.doi	10.3389/fnana.2016.00047	de
jgu.organisation.ror	https://ror.org/023b0x485	-
Appears in collections:	DFG-OA-Publizieren (2012 - 2017)

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