Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-7396
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dc.contributor.authorFreese, Christian-
dc.contributor.authorSchreiner, Daniel-
dc.contributor.authorAnspach, Laura-
dc.contributor.authorBantz, Christoph-
dc.contributor.authorMaskos, Michael-
dc.contributor.authorUnger, Ronald E.-
dc.contributor.authorKirkpatrick, Charles James-
dc.date.accessioned2022-07-13T09:47:03Z-
dc.date.available2022-07-13T09:47:03Z-
dc.date.issued2014
dc.identifier.urihttps://openscience.ub.uni-mainz.de/handle/20.500.12030/7410-
dc.description.abstractBACKGROUND In general the prediction of the toxicity and therapeutic efficacy of engineered nanoparticles in humans is initially determined using in vitro static cell culture assays. However, such test systems may not be sufficient for testing nanoparticles intended for intravenous application. Once injected, these nanoparticles are caught up in the blood stream in vivo and are therefore in continuous movement. Physical forces such as shear stress and cyclic stretch caused by the pulsatile blood flow are known to change the phenotype of endothelial cells which line the luminal side of the vasculature and thus may be able to affect cell-nanoparticle interactions. METHODS In this study we investigated the uptake of amorphous silica nanoparticles in primary endothelial cells (HUVEC) cultured under physiological cyclic stretch conditions (1 Hz, 5% stretch) and compared this to cells in a standard static cell culture system. The toxicity of varying concentrations was assessed using cell viability and cytotoxicity studies. Nanoparticles were also characterized for the induction of an inflammatory response. Changes to cell morphology was evaluated in cells by examining actin and PECAM staining patterns and the amounts of nanoparticles taken up under the different culture conditions by evaluation of intracellular fluorescence. The expression profile of 26 stress-related was determined by microarray analysis. RESULTS The results show that cytotoxicity to endothelial cells caused by silica nanoparticles is not significantly altered under stretch compared to static culture conditions. Nevertheless, cells cultured under stretch internalize fewer nanoparticles. The data indicate that the decrease of nanoparticle content in stretched cells was not due to the induction of cell stress, inflammation processes or an enhanced exocytosis but rather a result of decreased endocytosis. CONCLUSIONS In conclusion, this study shows that while the toxic impact of silica nanoparticles is not altered by stretch this dynamic model demonstrates altered cellular uptake of nanoparticles under physiologically relevant in vitro cell culture models. In particular for the development of nanoparticles for biomedical applications such improved in vitro cell culture models may play a pivotal role in the reduction of animal experiments and development costs.de_DE
dc.description.sponsorshipDFG, Open Access-Publizieren Universität Mainz / Universitätsmedizinde
dc.language.isoengde
dc.rightsCC BY*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subject.ddc610 Medizinde_DE
dc.subject.ddc610 Medical sciencesen_GB
dc.titleIn vitro investigation of silica nanoparticle uptake into human endothelial cells under physiological cyclic stretchde_DE
dc.typeZeitschriftenaufsatzde
dc.identifier.doihttp://doi.org/10.25358/openscience-7396-
jgu.type.dinitypearticleen_GB
jgu.type.versionPublished versionde
jgu.type.resourceTextde
jgu.organisation.departmentFB 04 Medizinde
jgu.organisation.number2700-
jgu.organisation.nameJohannes Gutenberg-Universität Mainz-
jgu.rights.accessrightsopenAccess-
jgu.journal.titleParticle and fibre toxicologyde
jgu.journal.volume11de
jgu.pages.alternativeArt. 68de
jgu.publisher.year2014-
jgu.publisher.nameBioMed centralde
jgu.publisher.placeLondonde
jgu.publisher.urihttps://doi.org/10.1186/s12989-014-0068-yde
jgu.publisher.issn1743-8977de
jgu.identifier.wos000349188900001
jgu.organisation.placeMainz-
jgu.subject.ddccode610de
opus.date.modified2018-08-09T08:25:12Z
opus.subject.dfgcode00-000
opus.organisation.stringFB 04: Medizin: Institut für Pathologiede_DE
opus.identifier.opusid50543
opus.importsourceWoS
opus.institute.number0423
opus.metadataonlyfalse
opus.type.contenttypeKeinede_DE
opus.type.contenttypeNoneen_EN
opus.affiliatedFreese, Christian
opus.affiliatedUnger, Ronald E.
opus.affiliatedKirkpatrick, Charles James
jgu.publisher.doi10.1186/s12989-014-0068-yde
jgu.organisation.rorhttps://ror.org/023b0x485-
Appears in collections:DFG-OA-Publizieren (2012 - 2017)

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