Gutenberg Open Science: Bacteriome-localized intracellular symbionts in pollen-feeding beetles of the genus dasytes (Coleoptera, Dasytidae)

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-7369

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DC Field	Value	Language
dc.contributor.author	Weiss, Benjamin	-
dc.contributor.author	Kaltenpoth, Martin	-
dc.date.accessioned	2022-07-12T07:42:57Z	-
dc.date.available	2022-07-12T07:42:57Z	-
dc.date.issued	2016	-
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/7383	-
dc.description.abstract	Several insect taxa are associated with intracellular symbionts that provision limiting nutrients to their hosts. Such tightly integrated symbioses are especially common in insects feeding on nutritionally challenging diets like phloem sap or vertebrate blood, but also occur in seed-eating and omnivorous taxa. Here, we characterize an intracellular symbiosis in pollen-feeding beetles of the genus Dasytes (Coleoptera, Dasytidae). High-throughput tag-encoded 16S amplicon pyrosequencing of adult D. plumbeus and D. virens revealed a single gamma-proteobacterial symbiont (‘Candidatus Dasytiphilus stammeri’) that amounts to 52.4–98.7% of the adult beetles’ entire microbial community. Almost complete 16S rRNA sequences phylogenetically placed the symbiont into a clade comprising Buchnera and other insect endosymbionts, but sequence similarities to these closest relatives were surprisingly low (83.4–87.4%). Using histological examination, three-dimensional reconstructions, and fluorescence in situ hybridization, we localized the symbionts in three mulberry-shaped bacteriomes that are associated with the mid- to hind-gut transition in adult male and female beetles. Given the specialized pollen-feeding habits of the adults that contrasts with the larvae’s carnivorous lifestyle, the symbionts may provision limiting essential amino acids or vitamins as in other intracellular symbioses, or they might produce digestive enzymes that break up the fastidious pollen walls and thereby contribute to the host’s nutrition. In either case, the presence of gamma-proteobacterial symbionts in pollen-feeding beetles indicates that intracellular mutualists are more widely distributed across insects with diverse feeding habits than previously recognized.	en_GB
dc.description.sponsorship	DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin	de
dc.language.iso	eng	de
dc.rights	CC BY	*
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	*
dc.subject.ddc	590 Tiere (Zoologie)	de_DE
dc.subject.ddc	590 Zoological sciences	en_GB
dc.title	Bacteriome-localized intracellular symbionts in pollen-feeding beetles of the genus dasytes (Coleoptera, Dasytidae)	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-7369	-
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 10 Biologie	de
jgu.organisation.number	7970	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	Frontiers in microbiology	de
jgu.journal.volume	7	de
jgu.pages.alternative	Art. 1486	de
jgu.publisher.year	2016	-
jgu.publisher.name	Frontiers Media	de
jgu.publisher.place	Lausanne	de
jgu.publisher.uri	http://dx.doi.org/10.3389/fmicb.2016.01486	de
jgu.publisher.issn	1664-302X	de
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	590	de
opus.date.modified	2018-08-22T10:10:57Z	-
opus.subject.dfgcode	00-000	-
opus.organisation.string	FB 10: Biologie: Institut für Zoologie	de_DE
opus.identifier.opusid	55147	-
opus.institute.number	1003	-
opus.metadataonly	false	-
opus.type.contenttype	Keine	de_DE
opus.type.contenttype	None	en_EN
opus.affiliated	Weiss, Benjamin	-
opus.affiliated	Kaltenpoth, Martin	-
jgu.publisher.doi	10.3389/fmicb.2016.01486	-
jgu.organisation.ror	https://ror.org/023b0x485
Appears in collections:	DFG-OA-Publizieren (2012 - 2017)

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