Gutenberg Open Science: Hydrogen sulfide protects retinal ganglion cells against glaucomatous injury in vitro and in vivo

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-7353

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DC Field	Value	Language
dc.contributor.author	Liu, Hanhan	-
dc.contributor.author	Anders, Fabian	-
dc.contributor.author	Thanos, Solon	-
dc.contributor.author	Mann, Carolina	-
dc.contributor.author	Liu, Aiwei	-
dc.contributor.author	Grus, Franz-Hermann	-
dc.contributor.author	Pfeiffer, Norbert	-
dc.contributor.author	Prokosch-Willing, Verena	-
dc.date.accessioned	2022-07-11T10:31:02Z	-
dc.date.available	2022-07-11T10:31:02Z	-
dc.date.issued	2017	-
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/7367	-
dc.description.abstract	Purpose: Hydrogen sulfide (H2S) is recognized as a novel third signaling molecule and gaseous neurotransmitter. Recently, cell protective properties within the central nervous and cardiovascular system have been proposed. Our purpose was to analyze the expression and neuroprotective effects of H2S in experimental models of glaucoma. Methods: Elevated IOP was induced in Sprague-Dawley rats by means of episcleral vein cauterization. After 7 weeks, animals were killed and the retina was analyzed with label-free mass spectrometry. In vitro, retinal explants were exposed to elevated hydrostatic pressure or oxidative stress (H2O2), with and without addition of a slow-releasing H2S donor Morpholin-4-ium-methoxyphenyl-morpholino-phosphinodithioate (GYY4137). In vivo, GYY4137 was injected intravitreally in animals with acute ischemic injury or optic nerve crush. Brn3a+ retinal ganglion cells (RGCs) were counted in retinal flat mounts and compared. Optical coherence tomography (OCT) was performed to examine the vessels. Comparisons were made by t-test and ANOVA (P < 0.05). Results: IOP elevation caused significant RGC loss (P < 0.001); 3-mercaptosulfurtransferase, an H2S producing enzyme, showed a 3-fold upregulation within the retina after IOP elevation. GYY4137 protected RGCs against elevated pressure and oxidative stress in vitro depending on the concentration used (P < 0.005). In vivo, intravitreal administration of GYY4137 preserved RGCs from acute ischemic injury and optic nerve crush (P < 0.0001). Retinal vessel diameters enlarged after intravitreal GYY4137 injection (P < 0.0001). Conclusions: H2S is specifically regulated in experimental glaucoma. By scavenging reactive oxygen species and dilating retinal vessels, H2S may protect RGCs from pressure and oxidative stress–induced RGC loss in vitro and in vivo. Therefore, H2S might be a novel neuroprotectant in glaucoma.	en_GB
dc.description.sponsorship	DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin	de
dc.language.iso	eng	de
dc.rights	CC BY-NC-ND	*
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject.ddc	610 Medizin	de_DE
dc.subject.ddc	610 Medical sciences	en_GB
dc.title	Hydrogen sulfide protects retinal ganglion cells against glaucomatous injury in vitro and in vivo	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-7353	-
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 04 Medizin	de
jgu.organisation.number	2700	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	Investigative ophthalmology & visual science	de
jgu.journal.volume	58	de
jgu.journal.issue	12	de
jgu.pages.start	5129	de
jgu.pages.end	5141	de
jgu.publisher.year	2017	-
jgu.publisher.name	ARVO	de
jgu.publisher.place	Rockville, Md.	de
jgu.publisher.uri	http://dx.doi.org/10.1167/iovs.17-22200	de
jgu.publisher.issn	1552-5783	de
jgu.publisher.issn	0146-0404	de
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	610	de
opus.date.modified	2018-03-16T10:12:31Z	-
opus.subject.dfgcode	00-000	-
opus.organisation.string	FB 04: Medizin: Augenklinik und Poliklinik	de_DE
opus.identifier.opusid	57987	-
opus.institute.number	0446	-
opus.metadataonly	false	-
opus.type.contenttype	Keine	de_DE
opus.type.contenttype	None	en_EN
opus.affiliated	Grus, Franz-Hermann	-
opus.affiliated	Pfeiffer, Norbert	-
opus.affiliated	Prokosch-Willing, Verena	-
jgu.publisher.doi	10.1167/iovs.17-22200	de
jgu.organisation.ror	https://ror.org/023b0x485
Appears in collections:	DFG-OA-Publizieren (2012 - 2017)

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