Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-7173
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DC Field | Value | Language |
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dc.contributor.author | Guggenhuber, Stephan | - |
dc.contributor.author | Romo-Parra, Hector | - |
dc.contributor.author | Bindila, Laura | - |
dc.contributor.author | Leschik, Julia | - |
dc.contributor.author | Lomazzo, Ermelinda | - |
dc.contributor.author | Remmers, Floortje | - |
dc.contributor.author | Zimmermann, Tina | - |
dc.contributor.author | Lerner, Raissa | - |
dc.contributor.author | Klugmann, Matthias | - |
dc.contributor.author | Pape, Hans-Christian | - |
dc.contributor.author | Lutz, Beat | - |
dc.date.accessioned | 2022-06-20T08:24:54Z | - |
dc.date.available | 2022-06-20T08:24:54Z | - |
dc.date.issued | 2016 | - |
dc.identifier.uri | https://openscience.ub.uni-mainz.de/handle/20.500.12030/7187 | - |
dc.description.abstract | Background: Postsynaptically generated 2-arachidonoylglycerol activates the presynaptic cannabinoid type-1 receptor, which is involved in synaptic plasticity at both glutamatergic and GABAergic synapses. However, the differential function of 2-arachidonoylglycerol signaling at glutamatergic vs GABAergic synapses in the context of animal behavior has not been investigated yet. Methods: Here, we analyzed the role of 2-arachidonoylglycerol signaling selectively in hippocampal glutamatergic neurons. Monoacylglycerol lipase, the primary degrading enzyme of 2-arachidonoylglycerol, is expressed at presynaptic sites of excitatory and inhibitory neurons. By adeno-associated virus-mediated overexpression of monoacylglycerol lipase in glutamatergic neurons of the mouse hippocampus, we selectively interfered with 2-arachidonoylglycerol signaling at glutamatergic synapses of these neurons. Results: Genetic modification of monoacylglycerol lipase resulted in a 50% decrease in 2-arachidonoylglycerol tissue levels without affecting the content of the second major endocannabinoid anandamide. A typical electrophysiological read-out for 2-arachidonoylglycerol signaling is the depolarization-induced suppression of excitation and of inhibition. Elevated monoacylglycerol lipase levels at glutamatergic terminals selectively impaired depolarization-induced suppression of excitation, while depolarization-induced suppression of inhibition was not significantly changed. At the behavioral level, mice with impaired hippocampal glutamatergic 2-arachidonoylglycerol signaling exhibited increased anxiety-like behavior but showed no alterations in aversive memory formation and seizure susceptibility. Conclusion: Our data indicate that 2-arachidonoylglycerol signaling selectively in hippocampal glutamatergic neurons is essential for the animal’s adaptation to aversive situations. | en_GB |
dc.description.sponsorship | DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin | de |
dc.language.iso | eng | de |
dc.rights | CC BY-NC | * |
dc.rights.uri | https://creativecommons.org/licenses/by-nc/4.0/ | * |
dc.subject.ddc | 610 Medizin | de_DE |
dc.subject.ddc | 610 Medical sciences | en_GB |
dc.title | Impaired 2-AG signaling in hippocampal glutamatergic neurons : aggravation of anxiety-like behavior and unaltered seizure susceptibility | en_GB |
dc.type | Zeitschriftenaufsatz | de |
dc.identifier.doi | http://doi.org/10.25358/openscience-7173 | - |
jgu.type.dinitype | article | en_GB |
jgu.type.version | Published version | de |
jgu.type.resource | Text | de |
jgu.organisation.department | FB 04 Medizin | de |
jgu.organisation.number | 2700 | - |
jgu.organisation.name | Johannes Gutenberg-Universität Mainz | - |
jgu.rights.accessrights | openAccess | - |
jgu.journal.title | The international journal of neuropsychopharmacology | de |
jgu.journal.volume | 19 | de |
jgu.journal.issue | 2 | de |
jgu.pages.alternative | pyv091 | de |
jgu.publisher.year | 2016 | - |
jgu.publisher.name | Oxford Univ. Press | de |
jgu.publisher.place | Oxford | de |
jgu.publisher.uri | http://dx.doi.org/10.1093/ijnp/pyv091 | de |
jgu.publisher.issn | 1469-5111 | de |
jgu.publisher.issn | 1461-1457 | de |
jgu.organisation.place | Mainz | - |
jgu.subject.ddccode | 610 | de |
opus.date.modified | 2017-05-12T07:16:32Z | - |
opus.subject.dfgcode | 00-000 | - |
opus.organisation.string | FB 04: Medizin: Institut für Physiologische Chemie und Pathobiochemie | de_DE |
opus.identifier.opusid | 56552 | - |
opus.institute.number | 0404 | - |
opus.metadataonly | false | - |
opus.type.contenttype | Keine | de_DE |
opus.type.contenttype | None | en_EN |
opus.affiliated | Guggenhuber, Stephan | - |
opus.affiliated | Bindila, Laura | - |
opus.affiliated | Leschik, Julia | - |
opus.affiliated | Lomazzo, Ermelinda | - |
opus.affiliated | Remmers, Floortje | - |
opus.affiliated | Zimmermann, Tina | - |
opus.affiliated | Lerner, Raissa | - |
opus.affiliated | Lutz, Beat | - |
jgu.publisher.doi | 10.1093/ijnp/pyv091 | de |
jgu.organisation.ror | https://ror.org/023b0x485 | |
Appears in collections: | DFG-OA-Publizieren (2012 - 2017) |
Files in This Item:
File | Description | Size | Format | ||
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impaired_2ag_signaling_in_hip-20220616160918345.pdf | 21.19 MB | Adobe PDF | View/Open |