Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-7169
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dc.contributor.authorSava, Bogdan A.-
dc.contributor.authorChen, Rongqing-
dc.contributor.authorSun, Haiyan-
dc.contributor.authorLuhmann, Heiko-
dc.contributor.authorKilb, Werner-
dc.date.accessioned2022-06-20T08:07:20Z-
dc.date.available2022-06-20T08:07:20Z-
dc.date.issued2014-
dc.identifier.urihttps://openscience.ub.uni-mainz.de/handle/20.500.12030/7183-
dc.description.abstractAlthough it has been suggested that taurine is the main endogenous neurotransmitter acting on glycine receptors, the implications of glycine receptor-mediated taurine actions on immature neocortical networks have not been addressed yet. To investigate the influence of taurine on the excitability of neuronal networks in the immature neocortex, we performed whole-cell patch-clamp recordings from visually identified pyramidal neurons and interneurons in coronal slices from C57Bl/6 and GAD67-green fluorescent protein (GFP) transgenic mice (postnatal days 2-4). In 46% of the pyramidal neurons bath-application of taurine at concentrations ≥ 300 μM significantly enhanced the frequency of postsynaptic currents (PSCs) by 744.3 ± 93.8% (n = 120 cells). This taurine-induced increase of PSC frequency was abolished by 0.2 μM tetrodotoxin (TTX), 1 μM strychnine or 3 μM gabazine, but was unaffected by the glutamatergic antagonists 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and (±) R(-)-3-(2-carboxypiperazine-4-yl)-propyl-1-phosphonic acid (CPP), suggesting that taurine specifically activates GABAergic network activity projecting to pyramidal neurons. Cell-attached recordings revealed that taurine enhanced the frequency of action potentials (APs) in pyramidal neurons, indicating an excitatory action of the GABAergic PSCs. In order to identify the presynaptic targets of taurine we demonstrate that bath application of taurine induced in GAD67-GFP labeled interneurons an inward current that is mainly mediated by glycine receptors and can generate APs in these cells. We conclude from these results that taurine can enhance network excitability in the immature neocortex by selectively activating GABAergic interneurons via interactions with glycine receptors.en_GB
dc.description.sponsorshipDFG, Open Access-Publizieren Universität Mainz / Universitätsmedizinde
dc.language.isoengde
dc.rightsCC BY*
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/*
dc.subject.ddc610 Medizinde_DE
dc.subject.ddc610 Medical sciencesen_GB
dc.titleTaurine activates GABAergic networks in the neocortex of immature miceen_GB
dc.typeZeitschriftenaufsatzde
dc.identifier.doihttp://doi.org/10.25358/openscience-7169-
jgu.type.dinitypearticleen_GB
jgu.type.versionPublished versionde
jgu.type.resourceTextde
jgu.organisation.departmentFB 04 Medizinde
jgu.organisation.number2700-
jgu.organisation.nameJohannes Gutenberg-Universität Mainz-
jgu.rights.accessrightsopenAccess-
jgu.journal.titleFrontiers in cellular neurosciencede
jgu.journal.volume8de
jgu.pages.alternativeArt. 26de
jgu.publisher.year2014-
jgu.publisher.nameFrontiers Research Foundationde
jgu.publisher.placeLausannede
jgu.publisher.urihttp://dx.doi.org/10.3389/fncel.2014.00026de
jgu.publisher.issn1662-5102de
jgu.organisation.placeMainz-
jgu.identifier.pmid24550782-
jgu.subject.ddccode610de
opus.date.modified2018-08-08T08:55:09Z-
opus.subject.dfgcode00-000-
opus.organisation.stringFB 04: Medizin: Institut für Physiologie und Pathophysiologiede_DE
opus.identifier.opusid27363-
opus.importsourcepubmed-
opus.institute.number0403-
opus.metadataonlyfalse-
opus.type.contenttypeKeinede_DE
opus.type.contenttypeNoneen_EN
opus.affiliatedLuhmann, Heiko-
jgu.publisher.doi10.3389/fncel.2014.00026de
jgu.organisation.rorhttps://ror.org/023b0x485
Appears in collections:DFG-OA-Publizieren (2012 - 2017)

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