Gutenberg Open Science: Redundant, exclusive, and cooperative functions of alternative exons in Drosophila Cav2 channels

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-7162

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DC Field	Value	Language
dc.contributor.author	Kilo, Lukas	-
dc.date.accessioned	2022-07-07T12:21:56Z	-
dc.date.available	2022-07-07T12:21:56Z	-
dc.date.issued	2022	-
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/7176	-
dc.description.abstract	The diversity of voltage gated calcium channel (VGCC) functions greatly outnumbers the count of voltage gated calcium channel genes. One of the ways to increase VGCC functional diversity is alternative splicing. In this thesis I could show for the Drosophila VGCC cacophony, a homolog to the vertebrate Cav2 calcium channel family, that functional diversity is indeed linked to alternative splicing. By using CRISPR/Cas for exon excision as well as electrophysiological methods, confocal microscopy, and behavioral assays, both isoform-specific properties and degenerate functions of exon dependent isoform subsets could be identified. Two alternative exon pairs encoding part of the voltage sensor or accessory subunit binding sites, respectively, were investigated. Differences in isoform localization as well as channel kinetics suggest a division of labor in some parts of the nervous system. Indeed, some of the isoforms fulfill essential functions with behavioral relevance, while others function in a degenerate manner. Although, whether these degenerate ones also exert unique functions under specific conditions remains to be determined. In addition to this, a novel collaborative interaction between two isoforms of the same gene could be discovered. Specifically, lethality resulting from the deletion of one essential isoform and behavioral impairments resulting from the deletion from the other alternative isoform are both fully rescued in trans heterozygous flies carrying both deletions. Both alternative exons have rather similar channel properties but different global and subcellular localizations. They are both required in concert throughout the nervous system for survival and normal motor behavior.	en_GB
dc.language.iso	eng	de
dc.rights	InCopyright	*
dc.rights.uri	http://rightsstatements.org/vocab/InC/1.0/	*
dc.subject.ddc	500 Naturwissenschaften	de_DE
dc.subject.ddc	500 Natural sciences and mathematics	en_GB
dc.subject.ddc	570 Biowissenschaften	de_DE
dc.subject.ddc	570 Life sciences	en_GB
dc.subject.ddc	590 Tiere (Zoologie)	de_DE
dc.subject.ddc	590 Zoological sciences	en_GB
dc.title	Redundant, exclusive, and cooperative functions of alternative exons in Drosophila Cav2 channels	en_GB
dc.type	Dissertation	de
dc.identifier.urn	urn:nbn:de:hebis:77-openscience-6d45d5b8-9ca1-4b89-bea7-5df7855f9a3e1	-
dc.identifier.doi	http://doi.org/10.25358/openscience-7162	-
jgu.type.dinitype	doctoralThesis	en_GB
jgu.type.version	Original work	de
jgu.type.resource	Text	de
jgu.date.accepted	2022-03	-
jgu.description.extent	VII, 130 Seiten, Illustrationen	de
jgu.organisation.department	FB 10 Biologie	de
jgu.organisation.year	2021	-
jgu.organisation.number	7970	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	500	de
jgu.subject.ddccode	570	de
jgu.subject.ddccode	590	de
jgu.organisation.ror	https://ror.org/023b0x485
Appears in collections:	JGU-Publikationen

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