Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-6952
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dc.contributor.authorMasomi-Bornwasser, Julia-
dc.contributor.authorWinter, Philipp-
dc.contributor.authorMüller-Werkmeister, Hendrik-
dc.contributor.authorStrand, Susanne-
dc.contributor.authorKönig, Jochem-
dc.contributor.authorKempski, Oliver-
dc.contributor.authorRingel, Florian-
dc.contributor.authorKantelhardt, Sven Rainer-
dc.contributor.authorKeric, Naureen-
dc.date.accessioned2022-05-09T12:38:42Z-
dc.date.available2022-05-09T12:38:42Z-
dc.date.issued2017
dc.identifier.urihttps://openscience.ub.uni-mainz.de/handle/20.500.12030/6964-
dc.description.abstractBackground Catheter-based lysis with recombinant tissue plasminogen activator (rtPA) is a well-established therapy for spontaneous intracerebral hemorrhage (ICH). The effectiveness of this therapy can be increased with ultrasound, but the optimal conditions are not yet clearly established. Using a novel in vitro system of blood clots previously developed by our group, we investigated various parameters of intralesional sonothrombolysis using an endosonography catheter in combination with rtPA. Methods Standardized human blood clots were equipped with a drainage catheter and weighed before and after 4 treatments: control (drainage only), rtPA only, ultrasound only and the combination of rtPA+ultrasound. The effectiveness of ultrasound was further analysed in terms of optimal frequency, duration and distance to the probe. Temperature and acoustic peak rarefaction pressure (APRP) were assessed to analyse potential adverse effects and quantify lysis. Histo-morphological analysis of the treated clots was performed by H&E staining and confocal laser scanning microscopy using fluorescent fibrinogen. Results The combined treatment rtPA+ultrasound achieved the highest lysis rates with a relative weight of 30.3%±5.5% (p≤0.0001) compared to all other groups. Similar results were observed when treating aged clots. Confocal fluorescent microscopy of the treated clots revealed a rarefied fibrin mesh without cavitations. No relevant temperature increase occurred (0.53±0.75°C). The optimal insonation treatment time was 1 hour. APRP measurements showed a lysis threshold of 515.5±113.4 kPa. Application of 10 MHz achieved optimal lysis and lysis radius, while simultaneously proving to be the best frequency for morphologic imaging of the clot and surrounding tissue. Conclusions These promising data provide the basis for an individualized minimal invasive ICH therapy by rtPA and sonothrombolysis independent of ICH age.de_DE
dc.description.sponsorshipDFG, Open Access-Publizieren Universität Mainz / Universitätsmedizinde
dc.language.isoengde
dc.rightsCC BY*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subject.ddc610 Medizinde_DE
dc.subject.ddc610 Medical sciencesen_GB
dc.titleCombination of ultrasound and rtPA enhances fibrinolysis in an In Vitro clot systemen_GB
dc.typeZeitschriftenaufsatzde
dc.identifier.doihttp://doi.org/10.25358/openscience-6952-
jgu.type.dinitypearticleen_GB
jgu.type.versionPublished versionde
jgu.type.resourceTextde
jgu.organisation.departmentFB 04 Medizinde
jgu.organisation.number2700-
jgu.organisation.nameJohannes Gutenberg-Universität Mainz-
jgu.rights.accessrightsopenAccess-
jgu.journal.titlePLOS ONEde
jgu.journal.volume12de
jgu.journal.issue11de
jgu.pages.alternativee0188131de
jgu.publisher.year2017-
jgu.publisher.namePLOSde
jgu.publisher.placeSan Francisco, California, USde
jgu.publisher.urihttp://dx.doi.org/10.1371/journal.pone.0188131de
jgu.publisher.issn1932-6203de
jgu.organisation.placeMainz-
jgu.subject.ddccode610de
opus.date.modified2019-08-06T10:05:17Z
opus.subject.dfgcode00-000
opus.organisation.stringFB 04: Medizin: Institut für Med. Biometrie, Epidemologie und Informatikde_DE
opus.organisation.stringFB 04: Medizin: I. Medizinische Klinik und Poliklinikde_DE
opus.organisation.stringFB 04: Medizin: Neurochirurgische Klinik und Poliklinikde_DE
opus.organisation.stringFB 04: Medizin: Institut für Neurochirurgische Pathophysiologiede_DE
opus.identifier.opusid57979
opus.institute.number0424
opus.institute.number0425
opus.institute.number0441
opus.institute.number0442
opus.metadataonlyfalse
opus.type.contenttypeKeinede_DE
opus.type.contenttypeNoneen_EN
opus.affiliatedStrand, Susanne
opus.affiliatedKönig, Jochem
opus.affiliatedKempski, Oliver
opus.affiliatedRingel, Florian
opus.affiliatedKantelhardt, Sven Rainer
opus.affiliatedKeric, Naureen
jgu.publisher.doi10.1371/journal.pone.0188131de
Appears in collections:DFG-OA-Publizieren (2012 - 2017)

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