Gutenberg Open Science: Combination of ultrasound and rtPA enhances fibrinolysis in an In Vitro clot system

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-6952

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DC Field	Value	Language
dc.contributor.author	Masomi-Bornwasser, Julia	-
dc.contributor.author	Winter, Philipp	-
dc.contributor.author	Müller-Werkmeister, Hendrik	-
dc.contributor.author	Strand, Susanne	-
dc.contributor.author	König, Jochem	-
dc.contributor.author	Kempski, Oliver	-
dc.contributor.author	Ringel, Florian	-
dc.contributor.author	Kantelhardt, Sven Rainer	-
dc.contributor.author	Keric, Naureen	-
dc.date.accessioned	2022-05-09T12:38:42Z	-
dc.date.available	2022-05-09T12:38:42Z	-
dc.date.issued	2017
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/6964	-
dc.description.abstract	Background Catheter-based lysis with recombinant tissue plasminogen activator (rtPA) is a well-established therapy for spontaneous intracerebral hemorrhage (ICH). The effectiveness of this therapy can be increased with ultrasound, but the optimal conditions are not yet clearly established. Using a novel in vitro system of blood clots previously developed by our group, we investigated various parameters of intralesional sonothrombolysis using an endosonography catheter in combination with rtPA. Methods Standardized human blood clots were equipped with a drainage catheter and weighed before and after 4 treatments: control (drainage only), rtPA only, ultrasound only and the combination of rtPA+ultrasound. The effectiveness of ultrasound was further analysed in terms of optimal frequency, duration and distance to the probe. Temperature and acoustic peak rarefaction pressure (APRP) were assessed to analyse potential adverse effects and quantify lysis. Histo-morphological analysis of the treated clots was performed by H&E staining and confocal laser scanning microscopy using fluorescent fibrinogen. Results The combined treatment rtPA+ultrasound achieved the highest lysis rates with a relative weight of 30.3%±5.5% (p≤0.0001) compared to all other groups. Similar results were observed when treating aged clots. Confocal fluorescent microscopy of the treated clots revealed a rarefied fibrin mesh without cavitations. No relevant temperature increase occurred (0.53±0.75°C). The optimal insonation treatment time was 1 hour. APRP measurements showed a lysis threshold of 515.5±113.4 kPa. Application of 10 MHz achieved optimal lysis and lysis radius, while simultaneously proving to be the best frequency for morphologic imaging of the clot and surrounding tissue. Conclusions These promising data provide the basis for an individualized minimal invasive ICH therapy by rtPA and sonothrombolysis independent of ICH age.	de_DE
dc.description.sponsorship	DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin	de
dc.language.iso	eng	de
dc.rights	CC BY	*
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	*
dc.subject.ddc	610 Medizin	de_DE
dc.subject.ddc	610 Medical sciences	en_GB
dc.title	Combination of ultrasound and rtPA enhances fibrinolysis in an In Vitro clot system	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-6952	-
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 04 Medizin	de
jgu.organisation.number	2700	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	PLOS ONE	de
jgu.journal.volume	12	de
jgu.journal.issue	11	de
jgu.pages.alternative	e0188131	de
jgu.publisher.year	2017	-
jgu.publisher.name	PLOS	de
jgu.publisher.place	San Francisco, California, US	de
jgu.publisher.uri	http://dx.doi.org/10.1371/journal.pone.0188131	de
jgu.publisher.issn	1932-6203	de
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	610	de
opus.date.modified	2019-08-06T10:05:17Z
opus.subject.dfgcode	00-000
opus.organisation.string	FB 04: Medizin: Institut für Med. Biometrie, Epidemologie und Informatik	de_DE
opus.organisation.string	FB 04: Medizin: I. Medizinische Klinik und Poliklinik	de_DE
opus.organisation.string	FB 04: Medizin: Neurochirurgische Klinik und Poliklinik	de_DE
opus.organisation.string	FB 04: Medizin: Institut für Neurochirurgische Pathophysiologie	de_DE
opus.identifier.opusid	57979
opus.institute.number	0424
opus.institute.number	0425
opus.institute.number	0441
opus.institute.number	0442
opus.metadataonly	false
opus.type.contenttype	Keine	de_DE
opus.type.contenttype	None	en_EN
opus.affiliated	Strand, Susanne
opus.affiliated	König, Jochem
opus.affiliated	Kempski, Oliver
opus.affiliated	Ringel, Florian
opus.affiliated	Kantelhardt, Sven Rainer
opus.affiliated	Keric, Naureen
jgu.publisher.doi	10.1371/journal.pone.0188131	de
jgu.organisation.ror	https://ror.org/023b0x485
Appears in collections:	DFG-OA-Publizieren (2012 - 2017)

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