Gutenberg Open Science: Npl3 stabilizes R-loops at telomeres to prevent accelerated replicative senescence

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-6239

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DC Field	Value	Language
dc.contributor.author	Pérez-Martínez, Lara	-
dc.contributor.author	Öztürk, Merve	-
dc.contributor.author	Butter, Falk	-
dc.contributor.author	Luke, Brian	-
dc.date.accessioned	2021-08-06T07:33:28Z	-
dc.date.available	2021-08-06T07:33:28Z	-
dc.date.issued	2020	-
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/6249	-
dc.description.abstract	Telomere shortening rates must be regulated to prevent premature replicative senescence. TERRA R-loops become stabilized at critically short telomeres to promote their elongation through homology-directed repair (HDR), thereby counteracting senescence onset. Using a non-bias proteomic approach to detect telomere binding factors, we identified Npl3, an RNA-binding protein previously implicated in multiple RNA biogenesis processes. Using chromatin immunoprecipitation and RNA immunoprecipitation, we demonstrate that Npl3 interacts with TERRA and telomeres. Furthermore, we show that Npl3 associates with telomeres in an R-loop-dependent manner, as changes in R-loop levels, for example, at short telomeres, modulate the recruitment of Npl3 to chromosome ends. Through a series of genetic and biochemical approaches, we reveal that Npl3 binds to TERRA and stabilizes R-loops at short telomeres, which in turn promotes HDR and prevents premature replicative senescence onset. This may have implications for diseases associated with excessive telomere shortening.	en_GB
dc.language.iso	eng	de
dc.rights	CC BY	*
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	*
dc.subject.ddc	570 Biowissenschaften	de_DE
dc.subject.ddc	570 Life sciences	en_GB
dc.title	Npl3 stabilizes R-loops at telomeres to prevent accelerated replicative senescence	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-6239	-
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 10 Biologie	de
jgu.organisation.number	7970	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	EMBO reports	de
jgu.journal.volume	21	de
jgu.journal.issue	3	de
jgu.pages.alternative	e49087	de
jgu.publisher.year	2020	-
jgu.publisher.name	Wiley	de
jgu.publisher.place	Hoboken, NJ u.a.	de
jgu.publisher.uri	https://doi.org/10.15252/embr.201949087	de
jgu.publisher.issn	1469-3178	de
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	570	de
jgu.publisher.doi	10.15252/embr.201949087
jgu.organisation.ror	https://ror.org/023b0x485
Appears in collections:	JGU-Publikationen

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