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http://doi.org/10.25358/openscience-5971Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Sudowe, Stephan | - |
| dc.contributor.author | Höhn, Yvonne | - |
| dc.contributor.author | Renzing, Andrea | - |
| dc.contributor.author | Maxeiner, Joachim | - |
| dc.contributor.author | Montermann, Evelyn | - |
| dc.contributor.author | Habermeier, Alice | - |
| dc.contributor.author | Closs, Ellen | - |
| dc.contributor.author | Bros, Matthias | - |
| dc.contributor.author | Reske-Kunz, Angelika B. | - |
| dc.date.accessioned | 2021-06-04T08:57:47Z | - |
| dc.date.available | 2021-06-04T08:57:47Z | - |
| dc.date.issued | 2020 | - |
| dc.identifier.uri | https://openscience.ub.uni-mainz.de/handle/20.500.12030/5980 | - |
| dc.description.abstract | We have previously shown that particle-mediated epidermal delivery (PMED) of plasmids encoding β-galactosidase (βGal) under control of the fascin-1 promoter (pFascin-βGal) yielded selective production of the protein in skin dendritic cells (DCs), and suppressed Th2 responses in a mouse model of type I allergy by inducing Th1/Tc1 cells. However, intranasal challenge of mice immunized with pFascin-βGal induced airway hyperreactivity (AHR) and neutrophilic inflammation in the lung. The tryptophan-catabolizing enzyme indoleamine 2,3-dioxygenase (IDO) has been implicated in immune suppression and tolerance induction. Here we investigated the consequences of co-application of an IDO-encoding vector on the modulatory effect of DNA vaccination by PMED using pFascin-βGal in models of eosinophilic allergic and non-eosinophilic intrinsic airway inflammation. IDO-encoding plasmids and pFascin-βGal or pCMV-βGal were co-applied to abdominal skin of BALB/c mice without, before or after sensitization with βGal protein. Immune responses in the lung were analysed after intranasal provocation and airway reactivity was determined by whole body plethysmography. Co-application of pCMV-IDO with pFascin-βGal, but not pCMV-βGal inhibited the Th1/Tc1 immune response after PMED. Moreover, AHR in those mice was attenuated following intranasal challenge. Therapeutic vaccination of βGal-sensitized mice with pFascin-βGal plus pCMV-IDO slightly suppressed airway inflammation and AHR after provocation with βGal protein, while prophylactic vaccination was not effective. Altogether, our data suggest that only the combination of DC-restricted antigen and ubiquitous IDO expression attenuated asthma responses in mice, most probably by forming a tryptophan-depleted and kynurenine-enriched micromilieu known to affect neutrophils and T cells. | en_GB |
| dc.language.iso | eng | de |
| dc.rights | CC BY | * |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | * |
| dc.subject.ddc | 610 Medizin | de_DE |
| dc.subject.ddc | 610 Medical sciences | en_GB |
| dc.title | Inhibition of antigen-specific immune responses by co-application of an indoleamine 2,3-dioxygenase (IDO)-encoding vector requires antigen transgene expression focused on dendritic cells | en_GB |
| dc.type | Zeitschriftenaufsatz | de |
| dc.identifier.doi | http://doi.org/10.25358/openscience-5971 | - |
| jgu.type.dinitype | article | en_GB |
| jgu.type.version | Published version | de |
| jgu.type.resource | Text | de |
| jgu.organisation.department | FB 04 Medizin | de |
| jgu.organisation.number | 2700 | - |
| jgu.organisation.name | Johannes Gutenberg-Universität Mainz | - |
| jgu.rights.accessrights | openAccess | - |
| jgu.journal.title | Amino acids | de |
| jgu.journal.volume | 52 | de |
| jgu.pages.start | 411 | de |
| jgu.pages.end | 424 | de |
| jgu.publisher.year | 2020 | - |
| jgu.publisher.name | Springer | de |
| jgu.publisher.place | Wien u.a. | de |
| jgu.publisher.uri | https://doi.org/10.1007/s00726-020-02817-4 | de |
| jgu.publisher.issn | 1438-2199 | de |
| jgu.organisation.place | Mainz | - |
| jgu.subject.ddccode | 610 | de |
| jgu.publisher.doi | 10.1007/s00726-020-02817-4 | |
| jgu.organisation.ror | https://ror.org/023b0x485 | |
| Appears in collections: | JGU-Publikationen | |
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| File | Description | Size | Format | ||
|---|---|---|---|---|---|
 | sudowe_stephan-inhibition_of_-20210527012518964.pdf | 1.29 MB | Adobe PDF | View/Open |