Gutenberg Open Science: Shikonin reduces growth of docetaxel-resistant prostate cancer cells mainly through necroptosis

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-5928

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DC Field	Value	Language
dc.contributor.author	Markowitsch, Sascha D.	-
dc.contributor.author	Juetter, Kira M.	-
dc.contributor.author	Schupp, Patricia	-
dc.contributor.author	Hauschulte, Kristine	-
dc.contributor.author	Vakhrusheva, Olesya	-
dc.contributor.author	Slade, Kimberly Sue	-
dc.contributor.author	Thomas, Anita	-
dc.contributor.author	Tsaur, Igor	-
dc.contributor.author	Cinatl, Jindrich	-
dc.contributor.author	Michaelis, Martin	-
dc.contributor.author	Efferth, Thomas	-
dc.contributor.author	Haferkamp, Axel	-
dc.contributor.author	Juengel, Eva	-
dc.date.accessioned	2021-05-31T09:49:25Z	-
dc.date.available	2021-05-31T09:49:25Z	-
dc.date.issued	2021	-
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/5937	-
dc.description.abstract	The prognosis for advanced prostate carcinoma (PCa) remains poor due to development of therapy resistance, and new treatment options are needed. Shikonin (SHI) from Traditional Chinese Medicine has induced antitumor effects in diverse tumor entities, but data related to PCa are scarce. Therefore, the parental (=sensitive) and docetaxel (DX)-resistant PCa cell lines, PC3, DU145, LNCaP, and 22Rv1 were exposed to SHI [0.1–1.5 μM], and tumor cell growth, proliferation, cell cycling, cell death (apoptosis, necrosis, and necroptosis), and metabolic activity were evaluated. Correspondingly, the expression of regulating proteins was assessed. Exposure to SHI time- and dose-dependently inhibited tumor cell growth and proliferation in parental and DX-resistant PCa cells, accompanied by cell cycle arrest in the G2/M or S phase and modulation of cell cycle regulating proteins. SHI induced apoptosis and more dominantly necroptosis in both parental and DX-resistant PCa cells. This was shown by enhanced pRIP1 and pRIP3 expression and returned growth if applying the necroptosis inhibitor necrostatin-1. No SHI-induced alteration in metabolic activity of the PCa cells was detected. The significant antitumor effects induced by SHI to parental and DX-resistant PCa cells make the addition of SHI to standard therapy a promising treatment strategy for patients with advanced PCa.	en_GB
dc.description.sponsorship	Open Access-Publizieren Universität Mainz / Universitätsmedizin Mainz	de
dc.language.iso	eng	de
dc.rights	CC BY	*
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	*
dc.subject.ddc	610 Medizin	de_DE
dc.subject.ddc	610 Medical sciences	en_GB
dc.title	Shikonin reduces growth of docetaxel-resistant prostate cancer cells mainly through necroptosis	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-5928	-
jgu.type.contenttype	Scientific article	de
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 04 Medizin	de
jgu.organisation.number	2700	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	Cancers	de
jgu.journal.volume	13	de
jgu.journal.issue	4	de
jgu.pages.alternative	882	de
jgu.publisher.year	2021	-
jgu.publisher.name	MDPI	de
jgu.publisher.place	Basel	de
jgu.publisher.uri	https://doi.org/10.3390/cancers13040882	de
jgu.publisher.issn	2072-6694	de
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	610	de
jgu.publisher.doi	10.3390/cancers13040882
jgu.organisation.ror	https://ror.org/023b0x485
Appears in collections:	JGU-Publikationen

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