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http://doi.org/10.25358/openscience-5760Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Imhof, Thomas | - |
| dc.contributor.author | Rosenblatt, Katharina | - |
| dc.contributor.author | Pryymachuk, Galyna | - |
| dc.contributor.author | Weiland, Daniela | - |
| dc.contributor.author | Noetzel, Nicolas | - |
| dc.contributor.author | Deschner, James | - |
| dc.contributor.author | Baris, Olivier R. | - |
| dc.contributor.author | Kimoloi, Sammy | - |
| dc.contributor.author | Koch, Manuel | - |
| dc.contributor.author | Wiesner, Rudolf J. | - |
| dc.contributor.author | Korkmaz, Yüksel | - |
| dc.date.accessioned | 2021-04-23T07:49:12Z | - |
| dc.date.available | 2021-04-23T07:49:12Z | - |
| dc.date.issued | 2020 | - |
| dc.identifier.uri | https://openscience.ub.uni-mainz.de/handle/20.500.12030/5769 | - |
| dc.description.abstract | The formation of dentin and enamel matrix depends on reciprocal interactions between epithelial-mesenchymal cells. To assess the role of mitochondrial function in amelogenesis and dentinogenesis, we studied postnatal incisor development in K320E-TwinkleEpi mice. In these mice, a loss of mitochondrial DNA (mtDNA), followed by a severe defect in the oxidative phosphorylation system is induced specifically in Keratin 14 (K14+) expressing epithelial cells. Histochemical staining showed severe reduction of cytochrome c oxidase activity only in K14+ epithelial cells. In mutant incisors, H&E staining showed severe defects in the ameloblasts, in the epithelial cells of the stratum intermedium and the papillary cell layer, but also a disturbed odontoblast layer. The lack of amelogenin in the enamel matrix of K320E-TwinkleEpi mice indicated that defective ameloblasts are not able to form extracellular enamel matrix proteins. In comparison to control incisors, von Kossa staining showed enamel biomineralization defects and dentin matrix impairment. In mutant incisor, TUNEL staining and ultrastructural analyses revealed differentiation defects, while in hair follicle cells apoptosis is prevalent. We concluded that mitochondrial oxidative phosphorylation in epithelial cells of the developed incisor is required for Ca2+ homeostasis to regulate the formation of enamel matrix and induce the differentiation of ectomesenchymal cells into odontoblasts. | de_DE |
| dc.language.iso | eng | de |
| dc.rights | CC BY | * |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | * |
| dc.subject.ddc | 610 Medizin | de_DE |
| dc.subject.ddc | 610 Medical sciences | en_GB |
| dc.title | Epithelial loss of mitochondrial oxidative phosphorylation leads to disturbed enamel and impaired dentin matrix formation in postnatal developed mouse incisor | en_GB |
| dc.type | Zeitschriftenaufsatz | de |
| dc.identifier.doi | http://doi.org/10.25358/openscience-5760 | - |
| jgu.type.dinitype | article | en_GB |
| jgu.type.version | Published version | de |
| jgu.type.resource | Text | de |
| jgu.organisation.department | FB 04 Medizin | de |
| jgu.organisation.number | 2700 | - |
| jgu.organisation.name | Johannes Gutenberg-Universität Mainz | - |
| jgu.rights.accessrights | openAccess | - |
| jgu.journal.title | Scientific reports | de |
| jgu.journal.volume | 10 | de |
| jgu.pages.alternative | 22037 | de |
| jgu.publisher.year | 2020 | - |
| jgu.publisher.name | Macmillan Publishers Limited, part of Springer Nature | de |
| jgu.publisher.place | London | de |
| jgu.publisher.uri | https://doi.org/10.1038/s41598-020-77954-7 | de |
| jgu.publisher.issn | 2045-2322 | de |
| jgu.organisation.place | Mainz | - |
| jgu.subject.ddccode | 610 | de |
| jgu.publisher.doi | 10.1038/s41598-020-77954-7 | |
| jgu.organisation.ror | https://ror.org/023b0x485 | |
| Appears in collections: | JGU-Publikationen | |
Files in This Item:
| File | Description | Size | Format | ||
|---|---|---|---|---|---|
 | x_x-epithelial_los-20210420092121799.pdf | 3.2 MB | Adobe PDF | View/Open |