Gutenberg Open Science: Considerations for the analysis of small extracellular vesicles in physical exercice

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-5623

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DC Field	Value	Language
dc.contributor.author	Brahmer, Alexandra	-
dc.contributor.author	Neuberger, Elmo W. I.	-
dc.contributor.author	Simon, Perikles	-
dc.contributor.author	Krämer-Albers, Eva-Maria	-
dc.date.accessioned	2021-02-04T11:49:02Z	-
dc.date.available	2021-02-04T11:49:02Z	-
dc.date.issued	2020	-
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/5627	-
dc.description.abstract	Physical exercise induces acute physiological changes leading to enhanced tissue cross-talk and a liberation of extracellular vesicles (EVs) into the circulation. EVs are cell-derived membranous entities which carry bioactive material, such as proteins and RNA species, and are important mediators of cell-cell-communication. Different types of physical exercise interventions trigger the release of diverse EV subpopulations, which are hypothesized to be involved in physiological adaptation processes leading to health benefits and longevity. Large EVs (“microvesicles” and “microparticles”) are studied frequently in the context of physical exercise using straight forward flow cytometry approaches. However, the analysis of small EVs (sEVs) including exosomes is hampered by the complex composition of blood, confounding the methodology of EV isolation and characterization. This mini review presents a concise overview of the current state of research on sEVs released upon physical exercise (ExerVs), highlighting the technical limits of ExerV analysis. The purity of EV preparations is highly influenced by the co-isolation of non-EV structures in the size range or density of EVs, such as lipoproteins and protein aggregates. Technical constraints associated with EV purification challenge the quantification of distinct ExerV populations, the identification of their cargo, and the investigation of their biological functions. Here, we offer recommendations for the isolation and characterization of ExerVs to minimize the effects of these drawbacks. Technological advances in the ExerV research field will improve understanding of the inter-cellular crosstalk induced by physical exercise leading to health benefits	en_GB
dc.description.sponsorship	DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin Mainz	de
dc.language.iso	eng	de
dc.rights	CC BY	*
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/deed.en	*
dc.subject.ddc	570 Biowissenschaften	de_DE
dc.subject.ddc	570 Life sciences	en_GB
dc.subject.ddc	796 Sport	de_DE
dc.subject.ddc	796 Athletic and outdoor sports and games	en_GB
dc.title	Considerations for the analysis of small extracellular vesicles in physical exercice	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-5623	-
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 02 Sozialwiss., Medien u. Sport	de
jgu.organisation.department	FB 10 Biologie	de
jgu.organisation.number	7910	-
jgu.organisation.number	7970	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	Frontiers in physiology	de
jgu.journal.volume	11	de
jgu.pages.alternative	576150	de
jgu.publisher.year	2020	-
jgu.publisher.name	Frontiers Research Foundation	de
jgu.publisher.place	Lausanne	de
jgu.publisher.uri	https://doi.org/10.3389/fphys.2020.576150	de
jgu.publisher.issn	1664-042X	de
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	570	de
jgu.subject.ddccode	796	de
jgu.publisher.doi	10.3389/fphys.2020.576150
jgu.organisation.ror	https://ror.org/023b0x485
Appears in collections:	JGU-Publikationen

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