Gutenberg Open Science: The absence of the drhm gene is not a marker for human‑pathogenicity in European Anaplasma phagocytophilum strains

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-5229

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DC Field	Value	Language
dc.contributor.author	Langenwalder, Denis B.	-
dc.contributor.author	Schmidt, Sabine	-
dc.contributor.author	Silaghi, Cornelia	-
dc.contributor.author	Skuballa, Jasmin	-
dc.contributor.author	Pantchev, Nikola	-
dc.contributor.author	Matei, Ioana A.	-
dc.contributor.author	Mihalca, Andrei D.	-
dc.contributor.author	Gilli, Urs	-
dc.contributor.author	Zajkowska, Joanna	-
dc.contributor.author	Ganter, Martin	-
dc.contributor.author	Hoffman, Tove	-
dc.contributor.author	Salaneck, Erik	-
dc.contributor.author	Petrovec, Miroslav	-
dc.contributor.author	Loewenich, Friederike D. von	-
dc.date.accessioned	2020-10-26T08:51:28Z	-
dc.date.available	2020-10-26T08:51:28Z	-
dc.date.issued	2020	-
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/5233	-
dc.description.abstract	Background: Anaplasma phagocytophilum is a Gram-negative obligate intracellular bacterium that replicates in neutrophil granulocytes. It is transmitted by ticks of the Ixodes ricinus complex and causes febrile illness in humans and animals. The geographical distribution of A. phagocytophilum spans the Americas, Europe, Africa and Asia. However, human disease predominantly occurs in North America but is infrequently reported from Europe and Asia. In North American strains, the absence of the drhm gene has been proposed as marker for pathogenicity in humans whereas no information on the presence or absence of the drhm gene was available for A. phagocytophilum strains circulating in Europe. Therefore, we tested 511 European and 21 North American strains for the presence of drhm and compared the results to two other typing methods: multilocus sequence typing (MLST) and ankA-based typing. Results: Altogether, 99% (478/484) of the analyzable European and 19% (4/21) of the North American samples from different hosts were drhm-positive. Regarding the strains from human granulocytic anaplasmosis cases, 100% (35/35) of European origin were drhm-positive and 100% (14/14) of North American origin were drhm-negative. Human strains from North America and Europe were both part of MLST cluster 1. North American strains from humans belonged to ankA gene clusters 11 and 12 whereas European strains from humans were found in ankA gene cluster 1. However, the North American ankA gene clusters 11 and 12 were highly identical at the nucleotide level to the European cluster 1 with 97.4% and 95.2% of identity, respectively. Conclusions: The absence of the drhm gene in A. phagocytophilum does not seem to be associated with pathogenicity for humans per se, because all 35 European strains of human origin were drhm-positive. The epidemiological differences between North America and Europe concerning the incidence of human A. phagocytophilum infection are not explained by strain divergence based on MLST and ankA gene-based typing. Keywords: Anaplasma phagocytophilum, ankA, APH_0919, APH_0922, Asia, drhm, Europe, Human, Multilocus sequence typing (MLST), North America, Pathogenicity	en_GB
dc.description.sponsorship	DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin	de
dc.language.iso	eng	de
dc.rights	CC BY	-
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	-
dc.subject.ddc	610 Medizin	de_DE
dc.subject.ddc	610 Medical sciences	en_GB
dc.title	The absence of the drhm gene is not a marker for human‑pathogenicity in European Anaplasma phagocytophilum strains	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-5229	-
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 04 Medizin	de
jgu.organisation.number	2700	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	Parasites & vectors	de
jgu.journal.volume	13	de
jgu.pages.alternative	Art. 238	de
jgu.publisher.year	2020	-
jgu.publisher.name	BioMed Central	de
jgu.publisher.place	London	de
jgu.publisher.uri	https://doi.org/10.1186/s13071-020-04116-z	de
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	610	de
jgu.publisher.doi	10.1186/s13071-020-04116-z	-
jgu.organisation.ror	https://ror.org/023b0x485	-
Appears in collections:	JGU-Publikationen

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