Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-2669
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dc.contributor.authorKulkarni, Apurv
dc.date.accessioned2018-05-14T07:19:50Z
dc.date.available2018-05-14T09:19:50Z
dc.date.issued2018
dc.identifier.urihttps://openscience.ub.uni-mainz.de/handle/20.500.12030/2671-
dc.description.abstractIn humans, the acetyltransferase MOZ ( a.k.a MYST3 and KAT6A) and its paralog MORF (a.k.a MYST4 and KAT6B) are known to function in a complex containing BRPF1 as a scaffold protein. Both MOZ/MORF and BRPF1 are known to play essential roles in embryonic CNS development, stem cell maintenance and adult neurogenesis in vertebrates. However, the molecular mechanism for these functions and the CNS specific acetylation targets of this complex remain unknown. Here, I show, that the fly homolog of MOZ/MORF , enok (enoki mushroom) is necessary for maintenance of a specific subpopulation of neuroblasts, termed the type II neuroblasts in the larval central brain. We show that conversion of type II neuroblasts to type I neuroblast fate accounts for this loss and this function of enok is dependent on the activity of the HAT complex. Interestingly, overexpression of enok leads to type I neuroblast converting to type II neuroblast fate. overexpression of enok also results in supernumerary Dpn+ neuroblast like cells in both type I and type II neuroblast lineages suggesting that progeny of enok overexpressing NB fail to differentiate. By performing ChIP-Seq and qRT-PCR analysis we show that enok binds to pnt and btd (two genes previously known to be necessary in type II neuroblast maintenance) loci and positively regulates their expression. Overexpression of pnt in enok knockdown background rescued the loss of type II neuroblasts suggesting that enok maintains type II neuroblasts by positively regulating its targets.en_GB
dc.language.isoeng
dc.rightsInCopyrightde_DE
dc.rights.urihttps://rightsstatements.org/vocab/InC/1.0/
dc.subject.ddc570 Biowissenschaftende_DE
dc.subject.ddc570 Life sciencesen_GB
dc.titleRole of enok in epigenetic gene regulation in neural stem cell development in Drosophila melanogasteren_GB
dc.typeDissertationde_DE
dc.identifier.urnurn:nbn:de:hebis:77-diss-1000019912
dc.identifier.doihttp://doi.org/10.25358/openscience-2669-
jgu.type.dinitypedoctoralThesis
jgu.type.versionOriginal worken_GB
jgu.type.resourceText
jgu.description.extent[12], 67 Blätter
jgu.organisation.departmentFB 10 Biologie-
jgu.organisation.year2018
jgu.organisation.number7970-
jgu.organisation.nameJohannes Gutenberg-Universität Mainz-
jgu.rights.accessrightsopenAccess-
jgu.organisation.placeMainz-
jgu.subject.ddccode570
opus.date.accessioned2018-05-14T07:19:50Z
opus.date.modified2018-05-28T10:35:54Z
opus.date.available2018-05-14T09:19:50
opus.subject.dfgcode00-000
opus.organisation.stringFB 10: Biologie: Institut für Genetikde_DE
opus.identifier.opusid100001991
opus.institute.number1005
opus.metadataonlyfalse
opus.type.contenttypeDissertationde_DE
opus.type.contenttypeDissertationen_GB
jgu.organisation.rorhttps://ror.org/023b0x485
Appears in collections:JGU-Publikationen

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