Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-2445
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dc.contributor.authorBoonrod, Kajohn-
dc.date.accessioned2002-12-31T23:00:00Z-
dc.date.available2003-01-01T00:00:00Z-
dc.date.issued2003-
dc.identifier.urihttps://openscience.ub.uni-mainz.de/handle/20.500.12030/2447-
dc.description.abstractExpression of antibodies in plant against essential viral proteins could provide an alternative approach to engineered viral resistance. Engineered single chain Fv antibodies scFV are particularly suitable for expression in plant because of their small size and the lack of assembly requirements. RNA-dependent RNA polymerases (RdRps) function as the catalytic subunit of viral replicases required for the replication of all positive strand RNA viruses. By using Phage technology we selected scFvs from a phage library using purified E.coli expressed TBSV(Tomato bushy stunt virus) replicase as antigen. The scFvs mediated-inhibition of RdRp activity was studied in vitro and in planta. In vitro experiments showed the inhibition of CNV(Cucumber necrosis virus) and TCV(Turnip crinkle virus) RdRp. Transient in planta assays based on agroinfiltration and an infectious clone of TBSV demonstrated the inhibition of the replication of TBSV(Tomato bushy stunt virus). Epitope mapping showed that the selected scFvs target the motif E of RdRp which is involved in template binding.Moreover T1 plants of transgenic lines of N. benthamiana expressing different scFvs either in the cytoplasm or the ER (endoplasmic reticulum) showed a high level of resistance against infection with TBSV and RCNMV(Red clover necrotic mosaic virus) upon inoculation with virus particles. This is the first report that scFvs against a RdRp of a plant viruses can inhibit viral replication in vivo. The resistance is even efficient against viruses belonging to different virus families.en_GB
dc.language.isoeng-
dc.rightsInCopyrightde_DE
dc.rights.urihttps://rightsstatements.org/vocab/InC/1.0/-
dc.subject.ddc570 Biowissenschaftende_DE
dc.subject.ddc570 Life sciencesen_GB
dc.titleA single chain antibody against a viral RNA polymerase (TBSV-BS3-Statice)en_GB
dc.typeDissertationde_DE
dc.identifier.urnurn:nbn:de:hebis:77-4573-
dc.identifier.doihttp://doi.org/10.25358/openscience-2445-
jgu.type.dinitypedoctoralThesis-
jgu.type.versionOriginal worken_GB
jgu.type.resourceText-
jgu.organisation.departmentFB 10 Biologie-
jgu.organisation.year2003-
jgu.organisation.number7970-
jgu.organisation.nameJohannes Gutenberg-Universität Mainz-
jgu.rights.accessrightsopenAccess-
jgu.organisation.placeMainz-
jgu.subject.ddccode570-
opus.date.accessioned2002-12-31T23:00:00Z-
opus.date.modified2004-08-04T11:05:35Z-
opus.date.available2003-01-01T00:00:00-
opus.organisation.stringFB 10: Biologie: FB 10: Biologiede_DE
opus.identifier.opusid457-
opus.institute.number1000-
opus.metadataonlyfalse-
opus.type.contenttypeDissertationde_DE
opus.type.contenttypeDissertationen_GB
jgu.organisation.rorhttps://ror.org/023b0x485
Appears in collections:JGU-Publikationen

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