Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-1428
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dc.contributor.authorBarbulescu, Karina
dc.date.accessioned1999-12-31T23:00:00Z
dc.date.available2000-01-01T00:00:00Z
dc.date.issued2000
dc.identifier.urihttps://openscience.ub.uni-mainz.de/handle/20.500.12030/1430-
dc.description.abstractInterferon-gamma is mainly produced by activated T helper cells and cytotoxic T lymphocytes and sustains the immune-defense against viral and bacterial infections. For a better understanding of IFN-gamma promoter regulation in T cells, different DNA-binding motivs were examined. Hereby, a new motiv (-196 to -183) was identified, that binds to the transcription factor AP-1 in T helper cells and Jurkat T cells. This factor acts as an essential activator protein. Further investigation demonstrated that IL-12 and IL-18 induce different regulatory pathways. Both AP-1 and STAT-4 bindings at their cognate DNA elements (-196 to -183 and -224 to -215) are required for the IL-12 dependent activation whereas IL-18 causes direct activation via AP-1.Moreover, the TH2 cytokine IL-4 represses significantly the IFN-gamma promoter activity in CD4+ T cells. IL-4 induces GATA-3, that interacts with two DNA-motivs (-111 to -87) at the IFN-gamma promoter.Furthermore, transgenic mice were generated, yielding a human IFN-gamma promoter construct (410 bp) under the control of a luciferase reporter gene. The data demonstrated a specific IFN-gamma promoter activation by antiCD3 plus antiCD28 in CD4+ and CD8+ T cells. The luciferase activty in CD4+ T cells was reinforced by addition of IL-12 and IL-18 and repressed by IL-4.en_GB
dc.language.isoger
dc.rightsin Copyrightde_DE
dc.rights.urihttps://rightsstatements.org/vocab/InC/1.0/
dc.subject.ddc570 Biowissenschaftende_DE
dc.subject.ddc570 Life sciencesen_GB
dc.titleTranskriptionelle Regulation des humanen Interferon-gamma-Promotors in T-Lymphocytende_DE
dc.typeDissertationde_DE
dc.identifier.urnurn:nbn:de:hebis:77-353
dc.identifier.doihttp://doi.org/10.25358/openscience-1428-
jgu.type.dinitypedoctoralThesis
jgu.type.versionOriginal worken_GB
jgu.type.resourceText
jgu.organisation.departmentFB 10 Biologie-
jgu.organisation.year2000
jgu.organisation.number7970-
jgu.organisation.nameJohannes Gutenberg-Universität Mainz-
jgu.rights.accessrightsopenAccess-
jgu.organisation.placeMainz-
jgu.subject.ddccode570
opus.date.accessioned1999-12-31T23:00:00Z
opus.date.modified1999-12-31T23:00:00Z
opus.date.available2000-01-01T00:00:00
opus.organisation.stringFB 10: Biologie: FB 10: Biologiede_DE
opus.identifier.opusid35
opus.institute.number1000
opus.metadataonlyfalse
opus.type.contenttypeDissertationde_DE
opus.type.contenttypeDissertationen_GB
Appears in collections:JGU-Publikationen

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