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http://doi.org/10.25358/openscience-1428
Autoren: | Barbulescu, Karina |
Titel: | Transkriptionelle Regulation des humanen Interferon-gamma-Promotors in T-Lymphocyten |
Online-Publikationsdatum: | 1-Jan-2000 |
Erscheinungsdatum: | 2000 |
Sprache des Dokuments: | Deutsch |
Zusammenfassung/Abstract: | Interferon-gamma is mainly produced by activated T helper cells and cytotoxic T lymphocytes and sustains the immune-defense against viral and bacterial infections. For a better understanding of IFN-gamma promoter regulation in T cells, different DNA-binding motivs were examined. Hereby, a new motiv (-196 to -183) was identified, that binds to the transcription factor AP-1 in T helper cells and Jurkat T cells. This factor acts as an essential activator protein. Further investigation demonstrated that IL-12 and IL-18 induce different regulatory pathways. Both AP-1 and STAT-4 bindings at their cognate DNA elements (-196 to -183 and -224 to -215) are required for the IL-12 dependent activation whereas IL-18 causes direct activation via AP-1.Moreover, the TH2 cytokine IL-4 represses significantly the IFN-gamma promoter activity in CD4+ T cells. IL-4 induces GATA-3, that interacts with two DNA-motivs (-111 to -87) at the IFN-gamma promoter.Furthermore, transgenic mice were generated, yielding a human IFN-gamma promoter construct (410 bp) under the control of a luciferase reporter gene. The data demonstrated a specific IFN-gamma promoter activation by antiCD3 plus antiCD28 in CD4+ and CD8+ T cells. The luciferase activty in CD4+ T cells was reinforced by addition of IL-12 and IL-18 and repressed by IL-4. |
DDC-Sachgruppe: | 570 Biowissenschaften 570 Life sciences |
Veröffentlichende Institution: | Johannes Gutenberg-Universität Mainz |
Organisationseinheit: | FB 10 Biologie |
Veröffentlichungsort: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-1428 |
URN: | urn:nbn:de:hebis:77-353 |
Version: | Original work |
Publikationstyp: | Dissertation |
Nutzungsrechte: | Urheberrechtsschutz |
Informationen zu den Nutzungsrechten: | https://rightsstatements.org/vocab/InC/1.0/ |
Enthalten in den Sammlungen: | JGU-Publikationen |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | ||
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![]() | 35.pdf | 17.6 MB | Adobe PDF | Öffnen/Anzeigen |