Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-9966Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Chan, Ya-Chien | - |
| dc.contributor.author | Kienle, Eike | - |
| dc.contributor.author | Oti, Martin | - |
| dc.contributor.author | Di Liddo, Antonella | - |
| dc.contributor.author | Mendez-Lago, Maria | - |
| dc.contributor.author | Aschauer, Dominik F. | - |
| dc.contributor.author | Peter, Manuel | - |
| dc.contributor.author | Pagani, Michaela | - |
| dc.contributor.author | Arnold, Cosmas | - |
| dc.contributor.author | Vonderheit, Andreas | - |
| dc.contributor.author | Schön, Christian | - |
| dc.contributor.author | Kreuz, Sebastian | - |
| dc.contributor.author | Stark, Alexander | - |
| dc.contributor.author | Rumpel, Simon | - |
| dc.date.accessioned | 2024-01-24T11:23:23Z | - |
| dc.date.available | 2024-01-24T11:23:23Z | - |
| dc.date.issued | 2023 | - |
| dc.identifier.uri | https://openscience.ub.uni-mainz.de/handle/20.500.12030/9984 | - |
| dc.description.abstract | Enhancers are important cis-regulatory elements controlling cell-type specific expression patterns of genes. Furthermore, combinations of enhancers and minimal promoters are utilized to construct small, artificial promoters for gene delivery vectors. Large-scale functional screening methodology to construct genomic maps of enhancer activities has been successfully established in cultured cell lines, however, not yet applied to terminally differentiated cells and tissues in a living animal. Here, we transposed the Self-Transcribing Active Regulatory Region Sequencing (STARR-seq) technique to the mouse brain using adeno-associated-viruses (AAV) for the delivery of a highly complex screening library tiling entire genomic regions and covering in total 3 Mb of the mouse genome. We identified 483 sequences with enhancer activity, including sequences that were not predicted by DNA accessibility or histone marks. Characterizing the expression patterns of fluorescent reporters controlled by nine candidate sequences, we observed differential expression patterns also in sparse cell types. Together, our study provides an entry point for the unbiased study of enhancer activities in organisms during health and disease. | en_GB |
| dc.language.iso | eng | de |
| dc.rights | CC BY | * |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | * |
| dc.subject.ddc | 610 Medizin | de_DE |
| dc.subject.ddc | 610 Medical sciences | en_GB |
| dc.title | An unbiased AAV-STARR-seq screen revealing the enhancer activity map of genomic regions in the mouse brain in vivo | en_GB |
| dc.type | Zeitschriftenaufsatz | de |
| dc.identifier.doi | http://doi.org/10.25358/openscience-9966 | - |
| jgu.type.contenttype | Scientific article | de |
| jgu.type.dinitype | article | en_GB |
| jgu.type.version | Published version | de |
| jgu.type.resource | Text | de |
| jgu.organisation.department | FB 04 Medizin | de |
| jgu.organisation.number | 2700 | - |
| jgu.organisation.name | Johannes Gutenberg-Universität Mainz | - |
| jgu.rights.accessrights | openAccess | - |
| jgu.journal.title | Scientific reports | de |
| jgu.journal.volume | 13 | de |
| jgu.pages.alternative | 6745 | de |
| jgu.publisher.year | 2023 | - |
| jgu.publisher.name | Macmillan Publishers Limited, part of Springer Nature | de |
| jgu.publisher.place | London | de |
| jgu.publisher.issn | 2045-2322 | de |
| jgu.organisation.place | Mainz | - |
| jgu.subject.ddccode | 610 | de |
| jgu.publisher.doi | 10.1038/s41598-023-33448-w | de |
| jgu.organisation.ror | https://ror.org/023b0x485 | - |
| Appears in collections: | DFG-491381577-G | |
Files in This Item:
| File | Description | Size | Format | ||
|---|---|---|---|---|---|
 | an_unbiased_aavstarrseq_scree-20240123161223121.pdf | 3.91 MB | Adobe PDF | View/Open |