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http://doi.org/10.25358/openscience-9736Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Bronkhorst, Alfred W. | - |
| dc.contributor.author | Lee, Chop Y. | - |
| dc.contributor.author | Möckel, Martin M. | - |
| dc.contributor.author | Ruegenberg, Sabine | - |
| dc.contributor.author | Jesus Domingues, Antonio de | - |
| dc.contributor.author | Sadouki, Shéraz | - |
| dc.contributor.author | Piccinno, Rossana | - |
| dc.contributor.author | Sumiyosh, Tetsutaro | - |
| dc.contributor.author | Siomi, Mikiko C. | - |
| dc.contributor.author | Stelzl, Lukas | - |
| dc.contributor.author | Luck, Katja | - |
| dc.contributor.author | Ketting, René F. | - |
| dc.date.accessioned | 2023-12-11T09:56:29Z | - |
| dc.date.available | 2023-12-11T09:56:29Z | - |
| dc.date.issued | 2023 | - |
| dc.identifier.uri | https://openscience.ub.uni-mainz.de/handle/20.500.12030/9754 | - |
| dc.description.abstract | Piwi-interacting RNAs (piRNAs) direct PIWI proteins to transposons to silence them, thereby preserving genome integrity and fertility. The piRNA population can be expanded in the ping-pong amplification loop. Within this process, piRNA-associated PIWI proteins (piRISC) enter a membraneless organelle called nuage to cleave their target RNA, which is stimulated by Gtsf proteins. The resulting cleavage product gets loaded into an empty PIWI protein to form a new piRISC complex. However, for piRNA amplification to occur, the new RNA substrates, Gtsf-piRISC, and empty PIWI proteins have to be in physical proximity. In this study, we show that in silkworm cells, the Gtsf1 homolog BmGtsf1L binds to piRNA-loaded BmAgo3 and localizes to granules positive for BmAgo3 and BmVreteno. Biochemical assays further revealed that conserved residues within the unstructured tail of BmGtsf1L directly interact with BmVreteno. Using a combination of AlphaFold modeling, atomistic molecular dynamics simulations, and in vitro assays, we identified a novel binding interface on the BmVreteno-eTudor domain, which is required for BmGtsf1L binding. Our study reveals that a single eTudor domain within BmVreteno provides two binding interfaces and thereby interconnects piRNA-loaded BmAgo3 and BmGtsf1L. | en_GB |
| dc.language.iso | eng | de |
| dc.rights | CC BY | * |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | * |
| dc.subject.ddc | 570 Biowissenschaften | de_DE |
| dc.subject.ddc | 570 Life sciences | en_GB |
| dc.title | An extended Tudor domain within Vreteno interconnects Gtsf1L and Ago3 for piRNA biogenesis in Bombyx mori | en_GB |
| dc.type | Zeitschriftenaufsatz | de |
| dc.identifier.doi | http://doi.org/10.25358/openscience-9736 | - |
| jgu.type.dinitype | article | en_GB |
| jgu.type.version | Published version | de |
| jgu.type.resource | Text | de |
| jgu.organisation.department | FB 10 Biologie | de |
| jgu.organisation.number | 7970 | - |
| jgu.organisation.name | Johannes Gutenberg-Universität Mainz | - |
| jgu.rights.accessrights | openAccess | - |
| jgu.journal.title | The EMBO journal | de |
| jgu.journal.volume | 42 | de |
| jgu.pages.alternative | e114072 | de |
| jgu.publisher.year | 2023 | - |
| jgu.publisher.name | Wiley | de |
| jgu.publisher.place | Hoboken, NJ u.a. | de |
| jgu.publisher.issn | 0261-4189 | de |
| jgu.organisation.place | Mainz | - |
| jgu.subject.ddccode | 570 | de |
| jgu.publisher.doi | 10.15252/embj.2023114072 | de |
| jgu.organisation.ror | https://ror.org/023b0x485 | - |
| jgu.subject.dfg | Lebenswissenschaften | de |
| Appears in collections: | DFG-491381577-H | |
Files in This Item:
| File | Description | Size | Format | ||
|---|---|---|---|---|---|
 | an_extended_tudor_domain_with-20231211104349103.pdf | 8.4 MB | Adobe PDF | View/Open |