Gutenberg Open Science: ERK3/MAPK6 dictates CDC42/RAC1 activity and ARP2/3-dependent actin polymerization

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-9348

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DC Field	Value	Language
dc.contributor.author	Bogucka-Janczi, Katarzyna	-
dc.contributor.author	Harms, Gregory	-
dc.contributor.author	Coissieux, Marie-May	-
dc.contributor.author	Bentires-Alj, Mohamed	-
dc.contributor.author	Thiede, Bernd	-
dc.contributor.author	Rajalingam, Krishnaraj	-
dc.date.accessioned	2023-08-08T07:10:56Z	-
dc.date.available	2023-08-08T07:10:56Z	-
dc.date.issued	2023	-
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/9366	-
dc.description.abstract	The actin cytoskeleton is tightly controlled by RhoGTPases, actin binding-proteins and nucleation-promoting factors to perform fundamental cellular functions. We have previously shown that ERK3, an atypical MAPK, controls IL-8 production and chemotaxis (Bogueka et al., 2020). Here, we show in human cells that ERK3 directly acts as a guanine nucleotide exchange factor for CDC42 and phosphorylates the ARP3 subunit of the ARP2/3 complex at S418 to promote filopodia formation and actin polymerization, respectively. Consistently, depletion of ERK3 prevented both basal and EGF-dependent RAC1 and CDC42 activation, maintenance of F-actin content, filopodia formation, and epithelial cell migration. Further, ERK3 protein bound directly to the purified ARP2/3 complex and augmented polymerization of actin in vitro. ERK3 kinase activity was required for the formation of actin-rich protrusions in mammalian cells. These findings unveil a fundamentally unique pathway employed by cells to control actin-dependent cellular functions.	en_GB
dc.description.sponsorship	Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 491381577	de
dc.language.iso	eng	de
dc.rights	CC BY	*
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	*
dc.subject.ddc	610 Medizin	de_DE
dc.subject.ddc	610 Medical sciences	en_GB
dc.title	ERK3/MAPK6 dictates CDC42/RAC1 activity and ARP2/3-dependent actin polymerization	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-9348	-
jgu.type.contenttype	Scientific article	de
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 04 Medizin	de
jgu.organisation.number	2700	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	eLife	de
jgu.journal.volume	12	de
jgu.pages.alternative	e85167	de
jgu.publisher.year	2023	-
jgu.publisher.name	eLife Sciences Publications	de
jgu.publisher.place	Cambridge	de
jgu.publisher.issn	2050-084X	de
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	610	de
jgu.publisher.doi	10.7554/eLife.85167	de
jgu.organisation.ror	https://ror.org/023b0x485	-
jgu.subject.dfg	Naturwissenschaften	de
Appears in collections:	DFG-491381577-G

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