Gutenberg Open Science: Neofunctionalization of ciliary BBS proteins to nuclear roles is likely a frequent innovation across eukaryotes

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-9021

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DC Field	Value	Language
dc.contributor.author	Ewerling, Alexander	-
dc.contributor.author	Maissl, Vanessa	-
dc.contributor.author	Wickstead, Bill	-
dc.contributor.author	May-Simera, Helen Louise	-
dc.date.accessioned	2023-04-18T09:48:46Z	-
dc.date.available	2023-04-18T09:48:46Z	-
dc.date.issued	2023	-
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/9038	-
dc.description.abstract	The eukaryotic BBSome is a transport complex within cilia and assembled by chaperonin-like BBS proteins. Recent work indicates nuclear functions for BBS proteins in mammals, but it is unclear how common these are in extant proteins or when they evolved. We screened for BBS orthologues across a diverse set of eukaryotes, consolidated nuclear association via signal sequence predictions and permutation analysis, and validated nuclear localization in mammalian cells via fractionation and immunocytochemistry. BBS proteins are—with exceptions— conserved as a set in ciliated species. Predictions highlight five most likely nuclear proteins and suggest that nuclear roles evolved independently of nuclear access during mitosis. Nuclear localization was confirmed in human cells. These findings suggest that nuclear BBS functions are potentially not restricted tomammals, but may be a common frequently co-opted eukaryotic feature. Understanding the functional spectrum of BBS proteins will help elucidating their role in gene regulation, development, and disease.	en_GB
dc.language.iso	eng	de
dc.rights	CC BY-NC-ND	*
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject.ddc	570 Biowissenschaften	de_DE
dc.subject.ddc	570 Life sciences	en_GB
dc.title	Neofunctionalization of ciliary BBS proteins to nuclear roles is likely a frequent innovation across eukaryotes	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-9021	-
jgu.type.contenttype	Scientific article	de
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 10 Biologie	de
jgu.organisation.number	7970	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	iScience	de
jgu.journal.volume	4	de
jgu.journal.issue	26	de
jgu.pages.alternative	106410	de
jgu.publisher.year	2023	-
jgu.publisher.name	Elsevier	de
jgu.publisher.place	Amsterdam ; Bosten ; London ; New York ; Oxford ; Paris ; Philadelphia ; San Diego ; St. Louis	de
jgu.publisher.issn	2589-0042	de
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	570	de
jgu.publisher.doi	10.1016/ j.isci.2023.106410	de
jgu.organisation.ror	https://ror.org/023b0x485	-
jgu.subject.dfg	Lebenswissenschaften	de
Appears in collections:	DFG-491381577-G

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