Gutenberg Open Science: Chromatin compaction precedes apoptosis in developing neurons

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-8674

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DC Field	Value	Language
dc.contributor.author	Rose, Renata	-
dc.contributor.author	Peschke, Nicolas	-
dc.contributor.author	Nigi, Elena	-
dc.contributor.author	Gelléri, Márton	-
dc.contributor.author	Ritz, Sandra	-
dc.contributor.author	Cremer, Christoph	-
dc.contributor.author	Luhmann, Heiko J.	-
dc.contributor.author	Sinning, Anne	-
dc.date.accessioned	2023-02-10T11:07:11Z	-
dc.date.available	2023-02-10T11:07:11Z	-
dc.date.issued	2022	-
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/8690	-
dc.description.abstract	While major changes in cellular morphology during apoptosis have been well described, the subcellular changes in nuclear architecture involved in this process remain poorly understood. Imaging of nucleosomes in cortical neurons in vitro before and during apoptosis revealed that chromatin compaction precedes the activation of caspase-3 and nucleus shrinkage. While this early chromatin compaction remained unaffected by pharmacological blockade of the final execution of apoptosis through caspase-3 inhibition, interfering with the chromatin dynamics by modulation of actomyosin activity prevented apoptosis, but resulted in necrotic-like cell death instead. With super-resolution imaging at different phases of apoptosis in vitro and in vivo, we demonstrate that chromatin compaction occurs progressively and can be classified into five stages. In conclusion, we show that compaction of chromatin in the neuronal nucleus precedes apoptosis execution. These early changes in chromatin structure critically affect apoptotic cell death and are not part of the final execution of the apoptotic process in developing cortical neurons.	en_GB
dc.description.sponsorship	Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 491381577	de
dc.language.iso	eng	de
dc.rights	CC BY	*
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	*
dc.subject.ddc	610 Medizin	de_DE
dc.subject.ddc	610 Medical sciences	en_GB
dc.title	Chromatin compaction precedes apoptosis in developing neurons	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-8674	-
jgu.type.contenttype	Scientific article	de
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 04 Medizin	de
jgu.organisation.number	2700	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	Communications biology	de
jgu.journal.volume	5	de
jgu.pages.alternative	797	de
jgu.publisher.year	2022	-
jgu.publisher.name	Springer Nature	de
jgu.publisher.place	London	de
jgu.publisher.issn	2399-3642	de
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	610	de
jgu.publisher.doi	10.1038/s42003-022-03704-2	de
jgu.organisation.ror	https://ror.org/023b0x485	-
jgu.subject.dfg	Lebenswissenschaften	de
Appears in collections:	DFG-491381577-G

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