Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-10138
Authors: | Sun, Mo Deng, Jie Walther, Andreas |
Title: | Communication and cross-regulation between chemically fueled sender and receiver reaction networks |
Online publication date: | 1-Mar-2024 |
Year of first publication: | 2022 |
Language: | english |
Abstract: | Nature connects multiple fuel-driven chemical/enzymatic reaction networks (CRNs/ERNs) via cross-regulation to hierarchically control biofunctions for a tailored adaption in complex sensory landscapes. Herein, we introduce a facile example of communication and cross-regulation among two fuel-driven DNA-based ERNs regulated by a concatenated RNA transcription regulator. ERN1 (“sender”) is designed for the fuel-driven promoter formation for T7 RNA polymerase, which activates RNA transcription. The produced RNA can deactivate or activate DNA in ERN2 (“receiver”) by toehold-mediated strand displacement, leading to a communication between two ERNs. The RNA from ERN1 can repress or promote the fuel-driven state of ERN2; ERN2 in turn feedbacks to regulate the lifetime of ERN1. Furthermore, the incorporation of RNase H allows for RNA degradation and enables the autonomous recovery of ERN2. We believe that concatenation of multiple CRNs/ERNs provides a basis for the design of more elaborate autonomous regulatory mechanisms in systems chemistry and synthetic biology. |
DDC: | 540 Chemie 540 Chemistry and allied sciences |
Institution: | Johannes Gutenberg-Universität Mainz |
Department: | FB 09 Chemie, Pharmazie u. Geowissensch. |
Place: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-10138 |
Version: | Published version |
Publication type: | Zeitschriftenaufsatz |
Document type specification: | Scientific article |
License: | CC BY-NC |
Information on rights of use: | https://creativecommons.org/licenses/by-nc/4.0/ |
Journal: | Angewandte Chemie International Edition 62 2 |
Pages or article number: | e202214499 |
Publisher: | Wiley |
Publisher place: | Weinheim |
Issue date: | 2022 |
ISSN: | 1521-3773 |
Publisher DOI: | 10.1002/anie.202214499 |
Appears in collections: | DFG-491381577-H |
Files in This Item:
File | Description | Size | Format | ||
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communication_and_crossregula-20240301091614496.pdf | 5.41 MB | Adobe PDF | View/Open |