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http://doi.org/10.25358/openscience-8715| Autoren: | Groeer, Saskia Garni, Martina Samanta, Avik Walther, Andreas |
| Titel: | Insertion of 3D DNA origami nanopores into block copolymer vesicles |
| Online-Publikationsdatum: | 2-Feb-2023 |
| Erscheinungsdatum: | 2022 |
| Sprache des Dokuments: | Englisch |
| Zusammenfassung/Abstract: | Block copolymer-based polymersomes are important building blocks for the bottom-up design of protocells and are considered advantageous over liposomes due to their higher mechanical stability and chemical versatility. Endowing both types of vesicles with capabilities for transmembrane transport is important for creating nanoreactor functionality and has been achieved by insertion of protein nanopores, even into comparably thick polymersome membranes. Still, the design space for protein nanopores is limited and higher flexibility might be accessible by de novo design of DNA nanopores, which have thus far been limited largely to liposome systems. Here, we introduce the successful insertion of two different 3D DNA origami nanopores into PMOXA-b-PDMS-b-PMOXA polymersomes, and confirm pore formation by dye influx studies and microscopy. This research thus opens the further design space of this versatile class of large DNA origami nanopores for polymersome-based functional protocells. |
| DDC-Sachgruppe: | 540 Chemie 540 Chemistry and allied sciences |
| Veröffentlichende Institution: | Johannes Gutenberg-Universität Mainz |
| Organisationseinheit: | FB 09 Chemie, Pharmazie u. Geowissensch. |
| Veröffentlichungsort: | Mainz |
| ROR: | https://ror.org/023b0x485 |
| DOI: | http://doi.org/10.25358/openscience-8715 |
| Version: | Published version |
| Publikationstyp: | Zeitschriftenaufsatz |
| Weitere Angaben zur Dokumentart: | Scientific article |
| Nutzungsrechte: | CC BY-NC-ND |
| Informationen zu den Nutzungsrechten: | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Zeitschrift: | ChemSystemsChem 4 6 |
| Seitenzahl oder Artikelnummer: | 202200009 |
| Verlag: | John Wiley & Sons, Ltd |
| Verlagsort: | Weinheim |
| Erscheinungsdatum: | 2022 |
| ISSN: | 25704206 |
| DOI der Originalveröffentlichung: | 10.1002/syst.202200009 |
| Enthalten in den Sammlungen: | DFG-491381577-H |
Dateien zu dieser Ressource:
| Datei | Beschreibung | Größe | Format | ||
|---|---|---|---|---|---|
 | insertion_of_3d_dna_origami_n-20230131085430701.pdf | 4.64 MB | Adobe PDF | Öffnen/Anzeigen |