Autoren:	Leber, Nadine Zentel, Rudolf
Titel:	Improved SiRNA loading of cationic nanohydrogel particles by variation of crosslinking density
Online-Publikationsdatum:	20-Mai-2022
Erscheinungsdatum:	2020
Sprache des Dokuments:	Englisch
Zusammenfassung/Abstract:	This paper deals with a concept to improve the loading capability of cationic nanohydrogel particles with siRNA. For this purpose, a new tetrafunctional crosslinker is synthesized via a peptide coupling approach using lysine and spermine derivatives. Applying this four-arm crosslinker to the particle synthesis makes it possible to perform the crosslinking with an excess or a deficit of the crosslinker. This allows for varying numbers of cationic groups per cationic core and its crosslinking density while the stability of the carrier system remains. The obtained cationic nanohydrogel particles are narrowly distributed in size as determined by dynamic light scattering measurements. Zeta potential measurements confirm the cationic nature of these carriers (ζ > +30 mV) and the ability to form complex anionic siRNA (agarose gel electrophoresis). As a result, it becomes possible to increase the siRNA loading by a factor of four by varying the composition of the crosslinked core. Such an increased siRNA loading should lead to an improved therapeutic gene knockdown effect.
DDC-Sachgruppe:	540 Chemie 540 Chemistry and allied sciences
Veröffentlichende Institution:	Johannes Gutenberg-Universität Mainz
Organisationseinheit:	FB 09 Chemie, Pharmazie u. Geowissensch.
Veröffentlichungsort:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-7011
Version:	Published version
Publikationstyp:	Zeitschriftenaufsatz
Nutzungsrechte:	CC BY-NC
Informationen zu den Nutzungsrechten:	https://creativecommons.org/licenses/by-nc/4.0/
Zeitschrift:	Macromolecular chemistry and physics 221 2
Seitenzahl oder Artikelnummer:	1900298
Verlag:	Wiley-VCH
Verlagsort:	Weinheim
Erscheinungsdatum:	2020
ISSN:	1521-3935
DOI der Originalveröffentlichung:	10.1002/macp.201900298
Enthalten in den Sammlungen:	JGU-Publikationen